尼罗红染色法检测拟微绿球藻油脂含量

Optimization of Nile red dyeing conditions for Nannochloropsis oculata

尼罗红染色法是检测胞内油脂较灵敏的方法,为了获得用尼罗红染色法检测拟微绿球藻油脂的最优条件,本文对尼罗红染色法的染色条件进行了研究,结果显示:二甲基亚砜(DMSO)浓度为20%,预处理温度35℃,尼罗红浓度为1.5μg/mL,染色时间10 min时,可以较为准确的反应出拟微绿球藻胞内的油脂含量.

The Nile red fluorescent dye was used to examine the lipid content of Nannochloropsis oculata strain. In order to obtain the optimal dyeing parameters, the influence of volume percentage of DMSO, pretreatment temperature, the concentration and staining time of Nile red was explored. Meanwhile, the factors were optimized by the orthogonal experimental design.The results indicated that the optimum dyeing conditions for Nannochloropsis oculata strain were as follows： volume percentage of DMSO is 20 %, pretreatment temperature is 35 ℃, the concentration of Nile red was 1.5 μg·mL-1, and the staining time of Nile red is 10 min. It’s expected that the strategy presented in this study could be used for rapid determination of lipid content of Nannochloropsis oculata strain.