α-苦瓜籽蛋白对体外培养人胚泡滋养层和蜕膜细胞的影响

Effects of a-momorcharin on human trophoblast and deciduacells in vitro

通过观察α-苦瓜籽蛋白对体外培养的人胚泡滋养层细胞形态、HCG分泌功能的影响,以及对人蜕膜细胞形态改变,探讨了α-苦瓜籽蛋白抗早孕的细胞学作用机理。0.13,0.50,2.00,8.00 μg/mlα-苦瓜籽蛋白对滋养层细胞形态有不同程度的损伤,时间越长、浓度越大则损伤越重;滋养层细胞分泌HCG功能受到明显抑制,同时对蜕膜细胞也有一定的毒性作用。实验证明:α-苦瓜籽蛋白具有直接损伤滋养层细胞以及毒害蜕膜细胞而达到抗早孕的作用。

By observing the morphological changes of the human trophoblast and decidua cells as well as the HCG secretion by the trophoblast cells in vitro, we tried to elucidate the cytological mechanism of the anti- early pregnancy effect of α-momorcharin.The results showed that the treated trophoblast cells all had obvious morphological damage in different degrees after 24 h treatment with different concentrations of a-momorcharin (0.13, 0.50, 2.00 and 8.00μg / ml). The degrees of damage were dependent on the concentration and duration, and α-momorcharin showed an inhibitory effect on HCG secretion. The reduction of HCG secretion could be detected as early as6 h after the treatment and the cells almost lost their ability of secreting HCG after 24 h treatment. The decidua cells were also damaged by a-momorcharin, but they were not as sensitive as trophoblast cells.Our results proved that the a-momorcharin treatment could not only directly kill trophoblast cells, but also damage decidua cells. Therefore the α-momorcharin treatment can terminate early pregnancy.