利用分子标记分析22种水稻10个抗稻瘟病基因的基因型

Genotyping of 10 blast resistance genes in 22 rice varieties by molecular marker

为了明确已克隆的部分稻瘟病抗性基因在上海市主栽水稻品种以及新培育的水稻品系中的分布,选取22份水稻为材料,利用分子标记检测技术,对10个已被克隆的抗稻瘟病基因进行基因型鉴定及分析.结果表明,22种水稻均含Pi36、Pi37、Pi-d2、Pib抗性基因,而Pi-kh、Pi9、Pi2、Pi-ta、Pikm抗性基因在22种水稻中分布各有不同,所有被测水稻中均不携带Pi25抗性基因.

Genotyping of 10 blast resistance genes,which were cloned from 22 leading rice cultivars or new bred rice strains grown mainly in Shanghai,was carried out by using molecular marker detection technology for clearing these genes' distribution in the 22 rice cultivars or strains. The results showed that 22 rice cultivars or strains all contained rice blast resistance genes Pi36、Pi37、Pi-d2 and Pib,while the distributions of Pi-kh、Pi9、Pi2、Pi-ta and Pikm were different in above different rice cultivars or strains. And all the rice samples detected did not contain Pi25.