甲状腺癌中Bax、Survivin及Livin的表达及意义

Significance and expression of Bax, Survivin and Livin in thyroid carcinoma

目的探讨甲状腺癌中B细胞淋巴瘤-2相关X蛋白(Bax)、生存素(Survivin)及Livin的表达及意义。方法采用免疫组化法检测82例甲状腺癌组织及相应癌旁正常组织中Bax、Survivin及Livin的阳性表达,分析三者与临床病理参数的关系。结果甲状腺癌组织中Bax的阳性表达率明显低于癌旁正常组织(P﹤0.001),Survivin及Livin的阳性表达率明显高于癌旁正常组织(P﹤0.001)。Ⅲ~Ⅳ期甲状腺癌患者的Bax阳性表达率明显低于Ⅰ~Ⅱ期患者,有淋巴结转移患者的Bax阳性表达率明显低于无淋巴结转移患者(P﹤0.01)。Ⅲ~Ⅳ期甲状腺癌患者的Survivin及Livin阳性表达率明显高于Ⅰ~Ⅱ期患者,有淋巴结转移患者的Survivin及Livin阳性表达率明显高于无淋巴结转移患者(P﹤0.01)。甲状腺癌组织中Bax与Survivin的表达呈负相关(r=-0.435,P﹤0.01),Bax与Livin的表达也呈负相关(r=-0.551,P﹤0.01),Survivin与Livin的表达呈正相关(r=0.738,P﹤0.01)。结论Bax在甲状腺癌组织中低表达,Survivin及Livin在甲状腺癌组织中高表达,并与TNM分期及淋巴结转移密切相关,三者联合检测可能成为甲状腺癌预后的参考指标。

Objective To explore the significance and expression of B-cell lymphoma-2 associated X protein（Bax）,Survivin and Livin in thyroid carcinoma. Method The expression of Bax, Survivin and Livin in 82 cases of thyroid carcinoma and corresponding adjacent normal tissues were detected by immunohistochemical assay. The relationship between Bax, Survivin, Livin and clinical pathological parameters was analyzed. Result The positive expression rate of Bax in thyroid carcinoma was significantly lower than that in adjacent normal tissues（P〈0.001）. The positive expression rate of Survivin and Livin in thyroid carcinoma were significantly higher than that in adjacent normal tissues（P〈0.001）.Lower positive expression rate of Bax was observed in patients with stage III-IV disease compared with those in stage I-II（P〈0.01）, and it was lower in those with lymph node metastasis vs no metastasis（P〈0.01）. As for the positive expression of Survivin and Livin, higher levels were observed in patients with stage III-IV disease or lymph node metastasis compared with those in stage I-II or without lymph node metastasis（P〈0.01）. The expression of Bax in thyroid carcinoma was negatively correlated with Survivin（r=-0.435,P〈0.01）, and Bax was also negatively correlated with Livin（r=-0.551,P〈0.01）, while Survivin was positively associated with Livin（r=0.738,P〈0.01）. Conclusion Low expression of Bax,high expression of Survivin and Livin are found in thyroid carcinoma tissues, and is related to TNM stage and lymph node metastasis. The combined detection might be a reference index for prognosis of thyroid cancer.