聚肽/聚己内酯共混电纺纳米纤维的制备以及高效Thiol-Yne光化学表面改性

Fabrication of Electrospinning Polypeptide/Polycaprolactone Blend Nanofibers and Their Highly Effective Surface Modification by Thiol-Yne Photochemistry

合成聚肽(synthetic polypeptides)在靶向递送、组织工程和再生医学领域表现独特,但其僵硬的主链构象导致难以用电纺的方法制备聚肽纳米纤维,而且后续的表面改性通常会破坏纤维的形貌,因而限制了这类材料的应用.为此,使用聚肽和聚己内酯(PCL)共混电纺的方法制备了一类新型的纳米纤维,并利用巯-炔(Thiol-Yne)光点击反应快速、高效、无损伤地对其表面进行改性.采用扫描电镜(SEM)、傅里叶变换红外(FT-IR)光谱和X射线光电子能谱(XPS)对其进行表征,结果显示:共混纤维的直径和形貌受聚肽含量的影响,而Thiol-Yne表面改性和后处理对纤维的形貌没有影响,这说明所制备的纤维的稳定性很好;利用温和的Thiol-Yne光点击反应可以成功地将氨基引入到纤维表面.

Synthetic polypeptides are unique biomaterials in the field of drug/gene delivery,tissue engineering and regenerative medicine.But electrospinning nanofibers used for tissue engineering are hard to be fabricated as a result of the rigid backbone of polypeptides caused by their α-helix and β-sheet conformations.In addition,the subsequent surface modifications of electrospinning nanofibers probably destroy the nanofibers.Thus,the fabrication and application of the electrospinning nanofibers of synthetic polypeptides are limited.In this work,electrospinning polypeptide/polycaprolactone（PCL）blend nanofibers were fabricated and then undergone surface modification by thiol-yne click chemistry,a fast non-destructive and highly effective access.Results of scanning electron microscope（SEM）and Fourier transform-infrared（FT-IR）spectroscopy showed that diameter and orientation of the nanofibers were determined by the polypeptide contents,and the surface modification did not destroy the surface morphology.X-ray photoelectron spectroscopy（XPS）confirmed the amine modification of the surface by the gentle thiol-yne method.