三氯乙酸(TCA)提取法与微波提取法检测活性污泥中三磷酸腺苷(ATP)

Detecting adenosine triphosphate(ATP) in activated sludge by trichloroacetic acid(TCA) extraction and microwave extraction

【目的】针对活性污泥法中的重要参数ATP进行研究分析,通过在不同条件下检测污泥的活性,得出以ATP为指标的污泥活性状态,为准确判定活性污泥的活性提供依据。【方法】分别运用三氯乙酸(TCA)提取法及微波提取法检测活性污泥中的ATP,并对检测ATP的影响因素(TCA浓度、冰浴时间、p H、微波频率及时间等)进行探讨与优化。【结果】运用TCA提取法检测ATP时,在1.0%-7.0%的TCA体积百分数内,活性污泥中TCA最佳体积百分数为2.5%;在2-60 min的冰浴时间内,最佳冰浴时间为10 min;三羟甲基丙烷-乙二胺四乙酸(Tris-EDTA)缓冲液的最佳p H 7.5;运用微波提取法检测ATP适宜的微波辐射条件为:功率800 W,辐射时间15 s。【结论】TCA提取法和微波提取法均可以检测活性污泥中的ATP,但与微波提取法相比,TCA提取法更能保证从细胞内释放出来的ATP的完整性,因此TCA提取法更适合用于检测活性污泥中的ATP。

[Objective] To provide the basis for the activity of activated sludge, as an important index in activated sludge, adenosine triphosphate（ATP） is detected to study the activity of activated sludge in the research. [Methods] The research used the method of TCA and microwave to detect ATP in activated sludge. Influencing factors（such as the concentration of TCA, time of immerging in an ice bath, p H, frequency of microwave and treatment time with microwave） are also discussed. [Results] The optimal concentration of TCA in the activated sludge is 2.5% with the concentration of TCA of 1.0%-7.0%; during 2-60 min immerging time in an ice bath, the optimal time using ice bath is 10 min; the optimal p H of Tris-EDTA buffer is 7.5. The suitable conditions for the method of microwave are： 800 W and 15 s for microwave irradiation. [Conclusion] The method of TCA and microwave can detect ATP in activated sludge. However, compared with the method of microwave, the method of TCA can guarantee the integrity of ATP, thus, the method of TCA is more suitable for detecting ATP in activated sludge.