杂草环境下转EPSPS基因大豆NZL06-698的生态适应性研究

Ecological Adaptability of Glyphosate-resistant Transgenic Soybean NZL06-698 in Weed Environment

为研究转EPSPS基因大豆NZL06-698在杂草环境中的生态适应性,试验设置杂草处理模拟野外环境,研究转基因大豆NZL06-698(GT698)的生存竞争能力以及外源EPSPS蛋白表达情况。结果表明:在相同处理下转基因大豆GT698的株高(R8期)、百粒重显著高于亲本大豆蒙豆12(MD12),但单株产量、单株籽粒数、结实率显著低于亲本大豆,说明外源基因的存在并未增强转基因大豆的生存竞争力,且转基因大豆在野外的生存竞争能力与亲本大豆相比较弱。试验注意到,杂草环境对转EPSPS基因大豆的生长有明显的限制作用,杂草处理下转基因大豆的单株产量、单株籽粒数、百粒重、结实率等指标均显著低于对照处理。ELISA检测结果表明,转基因大豆叶片及籽粒中外源EPSPS蛋白在杂草处理及对照中均正常表达,且两种处理下的EPSPS蛋白表达量无显著差异。以上结果表明杂草环境中转基因大豆的EPSPS蛋白正常表达,正常提供抗草甘膦性状,但是外源基因的存在并没有增加转基因大豆的生存竞争能力,且转基因大豆的生长受到杂草环境的显著抑制,由此得出结论:与亲本大豆相比,转EPSPS基因大豆NZL06-698在野外环境中生态适应能力较弱。

To study the ecological adaptability of glyphosate-resistant transgenic soybean NZL06-698（GT698） in the wild,we set weed treatment to simulate field environment to discuss the survival competitive ability of transgenic soybean and exogenous EPSPS protein expression.The results showed that the plant height（R8）,100-grain weight of GT698 were significantly higher than those of parent soybean Mengdou12（MD12） under weed treatment and control,but the yield per plant,seed numbers per plant,and seed setting rate was significantly lower than that of MD12,which meant exogenous gene epsps did not enhance survival competitive ability of GT698.Compared to MD12,survival competitive ability of GT698 was weak.The study noted that weed condition limited the growth of GT698 significantly,yield per plant,seed numbers per plant,100-grain weight and seed setting rate of GT698 in weed treatment were significantly lower than those of under control.ELISA showed that exogenous EPSPS protein in the leaves and grains of GT698 expressed normally in weed treatment and control,and there was no significant difference of EPSPS protein expression between two treatments.The above results showed that the transgenic soybean weeds expressed EPSPS protein normally,which provided GT698 with glyphosate-resistant trait.However,the exogenous gene did not increase the survival competitive ability of transgenic soybean,and the growth of GT698 was inhibited by weed significantly.In all,we speculated the ecological adaptation ability of GT698 is weaker comparing to the parent soybean MD12.