基于GGE-biplot的大豆根瘤菌抗逆性资源筛选

Screening the Resistance Resources of Rhizobium japonicum Based on GGE-biplot

为了准确评价大豆根瘤菌在干旱及酸碱环境中的稳定性和适应性,采用GGE双标图对黑龙江省不同生态区分离、鉴定、纯化的7个大豆根瘤菌菌株分别进行耐旱性、耐酸碱性能力分析评价。结果表明:各供试菌株随着PEG6000浓度的增加,菌株生长量均呈现逐渐下降的趋势。GGE双标图分析表明,耐旱性强且稳定性较好的菌株为111-1;供试菌株在耐酸碱性上均有较大优势,菌株在pH3.0和pH12.0的环境条件下均能缓慢生长,并且均在pH9.0的环境条件下生长量最大。GGE双标图分析得出,耐酸性强且稳定性较好的菌株为112-2,耐碱性强且稳定性较好的菌株为111-3。该结果对适于黑龙江地区不同环境条件下大豆根瘤菌的应用具有重要的指导意义。

In order to evaluate the stability and adaptability of Rhizobium japonicum in drought and acid base environment,analysis the ability of 7 Rhizobium japonicum strains on drought,acid and alkali resistance through GGE-Biplot,the strains were separated,identified and purified from different ecological area in Heilongjiang province.The results showed that the growth of the strain gradually decreased with the increase of PEG6000 concentration.GGE-Biplot showed that 111-1 was with strong drought resistance and better stability.The tested strains had great advantage in acid alkali resistance,can grow slowly when pH3.0 and pH12.0,and have the most growth increment when pH9.0.GGE-Biplot also showed that 112-2 was with high acid resistance and good stability,as the mean while,111-3 was with high alkali resistance and good stability.The results are of important meaning to the application of Rhizobium japonicum in different environmental conditions in Heilongjiang province.