油点花的组织培养与快速繁殖

Tissue Culture and Rapid Propagation of Ledebouria socialis(Liliaceae)

以油点花Ledebouria socialis(Baker)Jessop的叶片、叶柄和假鳞茎为外植体,经表面消毒处理,接种于不同激素配比的MS培养基中,探讨油点花外植体诱导愈伤组织、丛生芽、不定根培养的最适培养基和培养条件,以获得大量种苗,为其人工种植及商业化水平发展提供参考依据。结果表明:不同外植体均能诱导愈伤组织的产生,且叶片外植体最易诱导;适合油点花愈伤组织诱导的最适培养基为MS+6-BA 2.0 mg·L-1+NAA 0.5 mg·L-1,诱导率为96.3%~98.8%;而MS+6-BA 1.0 mg·L-1+NAA 0.1 mg·L-1适宜于芽诱导培养,诱导率为91.1%;培养基MS+NAA 0.2 mg·L-1适宜生根及壮苗培养,生根率89.5%;练苗移栽,其平均成活率达86.7%以上。

The leaf blade,petiole and bulbaceous from Ledebouria socialis（ Baker） Jessop were used as explants,which were cultured on MS media supplemented with different hormones to establish a rapid propagation system.The results confirmed a reliable method,which could be used for mass propagation of L.socialis at the commercial level.The results showed that different explants could induce callus,while those from leaf blades were rather successful.The optimal medium for callus induction was MS＋6-BA 2. 0 mg·L-1＋NAA0. 5 mg·L-1,and the induction percentage was 96. 3%-98. 8%; MS＋6-BA 1. 0 mg·L-1＋NAA 0. 1 mg·L-1 were suitable for differentiation of adventitious buds,and with 91. 1% in induction percentage.The medium MS＋NAA 0. 2 mg · L-1 was proper for forming roots,and rooting rate could be up to 89. 5%. Transplanting experiment showed that the tissue cultured seedling grew with more than 86. 7% survival rate.