广金钱草花粉活力测定及贮藏方法

Vitality Determination and Storage Method for Pollen of Desmodium styracifolium

以广金钱草花粉为试验材料,用染色法和离体培养法研究广金钱草花粉活力最佳测定方法,并探讨花粉贮藏的适应条件。结果表明:醋酸洋红染色法能快速检测广金钱草花粉活力,但活力较离体培养法低19.07%。广金钱草花粉在15%蔗糖+0.01%H3BO3+0.09%Ca(NO3)2·4H2O+0.01%Mg SO4·7H2O的液体培养基上培养2 h萌发率最高(96.16%),花粉管长度达到541.72μm。花粉贮藏72~168 h,4℃干燥处理明显高于其它贮藏条件下的花粉活力。试验表明,离体培养法最适合广金钱草花粉活力测定,4℃干燥贮藏条件下有利于花粉活力保持。

The pollens of Desmodium styracifolium were employed as experimental material to study the effects of test methods and storage conditions on pollen vitality of D. styracifolium by staining method and culturation in vitro.The results showed that,the pollen vitality of D.styracifolium could be determined quickly by staining method,which was lower than that of culturation method in vitro. The optimal medium was15% sucrose ＋ 0. 01% H3 BO3＋ 0. 09% Ca（ NO3）2· 4 H2 O ＋ 0. 01% Mg SO4· 7 H2 O,in which the rate of germination was 96. 16%,length of pollen tube was 541. 72 μm; 72-168 hours storage under 4 ℃ dry condition,it was good for the vitality maintenance of D. styracifolium pollens. The vitality of pollens dried by silicone was higher than the moist pollens.Culturation method in vitro was the most suitable method for D.styracifolium pollen viability determination,4 ℃ dry condition contributes was suitable for maintaining the pollen vitality of D.styracifolium.