姜黄素作用于卵巢癌靶基因的生物信息学预测

Bioinformatics prediction analysis of curcumin in ovarian cancer

目的:探讨姜黄素作用于靶基因的情况,并通过生物信息学技术分析预测参与的生物过程、信号通路和蛋白互作网络,从而探讨姜黄素对卵巢癌的作用。方法:利用PharmGKB数据库提取姜黄素的作用靶点,采用STRING9.1对姜黄素相关基因表达产物进行蛋白-蛋白相互作用的网络分析,采用WebGestalt数据库进行Pathway通路分析及GO功能注释,采用cBioPortal数据库和Human Protein Atlas分析,探索基因在癌组织中的表达情况。结果:从PharmGKB提取了10个靶点,将STRING9.1筛选出来的结果去重复,取交集,共筛选出386个与上述靶点相互作用的蛋白。应用WebGestalt数据库进行Pathway分析,得出10条通路,GO注释分类结果表明这些基因富集于18类分子功能,筛选出上述10条通路中与癌症相关的4条通路,利用cBioPortal数据库,将作用于该4条通路的基因整合,取交集,共得到7个hub基因,查询7个hub基因在卵巢癌中基因变异情况主要为基因扩增和基因缺失。利用The hunmanprotain atlas在线数据库,我们发现MAPK1、MAPK3、MAPK8在卵巢癌组织中高表达。结论:姜黄素可能通过MAPK通路,对卵巢癌的发生、发展起到一定作用。

Objective：To search the target - gene of curcumin, by using bioinformatics to analysis the biological process, signaling pathway and protein -protein interaction network （PPI） ,we found curcumin could have a inhibitory effect for ovarian cancer. Methods：By using PharmGKB database,we extracted the target - genes of cureumin, analy- sis PPI network and GO and pathway through WebGestalt. Finally,we verified the related genes in cBioPortal and Hu- man Protein Arias database. Results： Ten target - gene were found in PharmGKB, and 386 PPI genes from STRING database. Ten pathways were explored through WebGestah, finally seven hub -gene were confirmed. Moreover, seven hub - genes were tested the mutations in the eBioPortal. And MAPK1, MAPK3, MAPK8 were greatly amplified in the ovarian cancer tissue. Conclusion：The curcumin may play a role in the progression of ovarian cancer through MAPK signaling pathway.