50份长果黄麻种质资源耐盐性鉴定评价

Evaluation for Salt Tolerance of 50 Jute(Corchorus olitorius L. )Germplasm Resources

通过对来自不同国家的50份长果黄麻种质苗期耐盐性综合评价,评估不同黄麻种质耐盐特性,筛选黄麻耐盐极端材料,为进一步挖掘黄麻耐盐基因及分子机理研究准备材料。本研究采用水培法,设0、250 mmol/L Na Cl两个浓度对50份黄麻种质材料进行处理,调查盐处理后每份材料盐害指数的变化以及第8天的死亡率,建立盐害指数随时间变化的回归模型,利用回归方程分别求出每份材料盐害症状出现的时间以及盐害指数达到50%的时间,通过主成分分析、隶属函数法、聚类分析等方法对供试材料进行综合评价和耐盐性级别划分。结果表明,250 mmol/L Na Cl胁迫对于本试验是一个适宜的浓度;建立的50个回归方程拟合效果良好;根据对50份黄麻种质材料的综合评价值及聚类分析,可以将其分为4种耐盐类型,其中,高耐盐材料3份,耐盐材料6份,盐敏感材料10份,中度耐盐材料31份。

The objective of this study was to comprehensively evaluate the salt tolerance of 50 jute germplasm resources（ Corchorus olitorius L. ）from different countries, to excavate their own salt tolerance genetic resources, to provide a theoretical basis for evaluation of amounts of jute germplasms on salt tolerance, and to provide basic mate- rims for salt-tolerance gene discovery and the study of molecular mechanism. In this study,50 jute germplasms were cultured with hydroponics supplemented with NaC1 at different concentrations of 0,250 mmol/L, repectively. Then the salt injury index after salt treatment and death rate on the eighth day（DR） of the 50 germplasms were investiga- ted. The regression equation models reflecting the correlation between stressed duration and salt injury index of jute germplasms were established, in order to get the time when the symptom of salt damage appeared （SA） and salt injury index reached 50% （SX）. And 50 jute germplasms were evaluated and classified accoding to salt resis- tence by principle components analysis, subordination function method and hierarchical cluster analysis. The result showed that 250 mmol/L NaC1 was a suitable concentration for screening salt tolerant materials in this experi- ment. 50 regression equations established demonstrated fitted well. Based on the comprehensive ability of salt tol- erance of materials and cluster analysis,50 jute germplasms could be classified into 4 grades. Three germplasms were highly salt tolerant,6 germplasms were salt tolerant, 31 germplasms were middle tolerant, and 10 materials were salt sensitive.