斯钙素1对卵巢上皮细胞恶性转化的影响

The effect of stanniocalcin-1 on the malignant transformation of ovarian epithelial cells

目的:探讨斯钙素1(STC1)对卵巢上皮细胞恶性转化的影响。方法:采用慢病毒转染技术将STC1基因导入永生化的卵巢上皮细胞(记作T29-STC1),转染空载体的永生化卵巢上皮细胞(记作T29-Vector)作为对照。Western blot法检测T29-STC1和T29-Vector细胞中STC1表达;流式细胞术检测STC1表达对细胞周期的影响;细胞平板克隆实验和MTT法检测细胞增殖能力;接种裸鼠背部皮下移植瘤实验检测细胞成瘤能力。结果:T29-STC1细胞中STC1表达明显增强(P<0.05)。T29-STC1细胞中G0/G1期细胞数明显低于T29-Vector细胞(P<0.05),S期细胞数明显高于T29-Vector细胞(P<0.05)。T29-STC1细胞克隆形成数多于T29-Vector细胞(P<0.05),加卡铂处理细胞后,T29-STC1和T29-Vector的细胞克隆形成数均减少(P<0.05),但T29-Vector更显著。卡铂作用于细胞后,T29-STC1细胞与T29-Vector细胞相比生长速度明显增快(P<0.05);裸鼠体内,接种T29-STC1细胞可成瘤,而接种T29-Vector细胞不能成瘤。结论:STC1蛋白可能参与卵巢上皮细胞的恶性转化。

Objective： To study the effect of STC1 on the malignant transformation of ovarian epithelial cells.Methods： STC1 gene was transfected into immortalized ovarian epithelial cells（T29-STC1） by lentivirus transfection as study group,while control group（T29-Vector）was transfected with empty vector. Western blot was used to detect the expression of STC1 in T29-STC1 cells and T29-Vector cells. Cytometry was used to determine whether the level of STC1 expression affects cell cycle progression.Plate cloning experiments was used to count the number of cell formation after disposed by carboplatin.MTT assay was used to detect the proliferation ability of the cells and subcutaneous tumor xenograft in nude mice was used to detect the ability of tumor formation. Results： Western blot showed that the expression of STC1 in T29-STC1 was significantly enhanced.Cytometry showed that the T29-STC1 cells in G0 and G1 cell cycle decreased but increased in S cell cycle in comparision with T29-Vectorcells.Plate cloning experiments and MTT showed that the growth rate of T29-STC1 was significantly higher than that of T29-Vector after carboplatin treatment. T29-STC1 inoculated in nude mice could form tumor while T29-Vector could not.Conclusion： Stanniocalcin-1（STC1） protein may be involved in malignant transformation of ovarian epithelial cells.