7种生物碱在不同粒径卷烟主流烟气气溶胶中的分布研究

Distribution of 7 Alkaloids in Aerosol of Various Particle Sizes from Mainstream Cigarette Smoke

建立了烟气气溶胶中7种生物碱的气相色谱-质谱(GC-MS)测定方法,并采用电子低压撞击器(ELPI)分12级捕集烟气气溶胶粒相物,研究了卷烟主流烟气气溶胶中7种生物碱含量和浓度的粒径分布。捕集的气溶胶样品加入氢氧化钠溶液和二氯甲烷进行碱法提取,提取液经DB-5MS弹性毛细管柱分离,选择离子监测模式测定,内标法定量。结果表明:该法检测主流烟气气溶胶中7种生物碱的相对标准偏差(RSD)为2.1%~6.4%,检出限为0.39~14.84 ng/cig,加标回收率为85.5%~124.8%。7种生物碱主要分布于0.144~0.722μm的中等粒径气溶胶粒相物中,在粒径0.431μm的粒相物中含量最高,与捕集的粒相物质量分布一致。7种生物碱在不同粒径气溶胶粒相物中的浓度基本趋于一致,其浓度随气溶胶粒径的分布无特异性。

A method was developed for the analysis of 7 alkaloids in mainstream cigarette smoke aerosol by gas chromatography-mass spectrometry.By using electrical low-pressure impactor（ELPI）,particles of cigarette smoke aerosol were divided into 12 stages and collected.The content and concentration distributions of 7 alkaloids with respect to smoke aerosol particle size were investigated.The collected aerosol particles were treated by alkali extraction method with sodium hydroxide solution and dichloromethane.The extract was separated with a DB-5MS capillary column under selected ion monitoring（SIM） mode.The targets were quantitatively analyzed using internal standard method.The results showed that,the relative standard deviations（RSDs） for the established method ranged from 2.1%to 6.4%,with limits of detection（LODs） of 0.39-14.84 ng/cig,and the recoveries at three spiked levels ranged from 85.5%to 124.8%.All of 7 alkaloids mainly distributed in aerosol of medium size particles（0.144-0.722 μm） with a peak content at particle size of 0.431 μm,which was similar to the distribution pattern of collected particle mass.The concentrations of 7 alkaloids were uniform throughout the particles of various sizes,indicating a non-specificity distribution.