摘要
化学发光免疫分析技术凭借其化学发光的高灵敏性和免疫反应的高特异性在微生物快速检测中广泛应用。该文着重叙述了化学发光免疫分析技术核心检测体系在微生物检测中的研究进展,并对其涉及到的样品前处理和检测新方法进行了综述。化学发光免疫分析技术检测微生物用时短、成本低,但特异性识别方法和检测的灵敏度有待提升,新型的发光体系、发光放大方法、可替代抗体的识别分子以及相关的前处理技术是未来研究的重点。
Chemiluminescence immunoassay is widely used in the rapid detection of microbes because of its high sensitivity of chemiluminescence and high specificity of immune response.In the paper,the advance of the coresystems of chemiluminescence immunoassay in microbiological detection is emphatically illustrated.The related sample pretreatments and the new detection methods are reviewed.Although Chemiluminescence immunoassay in the microbiological detection has the advantages of rapidness and low consumption,its specific recognition and sensitivity could be further improved.The innovative luminescence systems,the luminescence amplification methods,the alternative recognition elements and the related pretreatment techniques are the focuses in future research.
出处
《分析测试学报》
CAS
CSCD
北大核心
2017年第11期1409-1416,共8页
Journal of Instrumental Analysis
基金
国家重大科学仪器设备开发专项(2013YQ140371)
国家重点研发计划资助(2016YFF0203802)
北京市科技计划项目(Z161100000916001)
北京市自然科学基金项目(2162018)
关键词
化学发光免疫分析技术
微生物检测
前处理
发光放大
识别分子
chemiluminescence immunoassay
microbiological detection
pretreatment
luminescence amplification
recognition element
