摘要
目的建立高效液相色谱法(high performance liquid chromatography,HPLC)同时测定墓头回药材中齐墩果酸和熊果酸含量的方法。方法采用HPLC色谱法,色谱柱为Sil Green C_(18)(5μm,4.6 mm×250 mm);以甲醇-0.2%乙酸铵水溶液(85∶15)为流动相,检测波长为210 nm,流速为1.0 ml/min,柱温为30℃。结果齐墩果酸和熊果酸分别在0.02510~1.0041mg/ml、0.02527~1.0109 mg/ml浓度范围内具有良好线性关系(r=0.99999),平均回收率分别为95.71%[相对标准偏差(relative standard deviation,RSD)=0.92%,n=6]、96.19%(RSD=1.16%,n=6)。结论 HPLC测定墓头回药材中齐墩果酸和熊果酸含量具有灵敏度高、快速、简便、专属性强、准确和重复性好等优点,可为墓头回药材质量控制标准的建立提供科学依据。
Objective The objective of this study was to establish a high performance liquid chromatography (HPLC) method for simultaneous measuring of the concentration of oleanolic acid and ursolic acid in P. heterophylla. Methods The analysis was performed on a SilGreen Cl8 (5μm,4.6 mm×250 mm) chromatographic column with mobile phase consisting of methanol - 0.2% ammonium acetate water (85 : 15). The detected wavelength was set at 210 nm. The flow rate was 1.0 ml/min and the column temperature was at 30℃. Results The linearity was good in the ranges of 0.02510-1.0041 mg/ml and 0.02527-1.0109 mg/ml respectively for oleanolic acid and ursolic acid (r=0.99999): The average recovery rates of oleanolic acid and ursolic acid were 95.71% [relative standard deviation (RSD=0.92%, n=6] and 96.19% (RSD=1.16%, n=6) respectively. Conclusions The method of HPLC features high sensitivity, time efficiency, simplicity, strong specificity, high accuracy and good repeatability, which can be used for the quality control of P. heterophylla.
出处
《中华灾害救援医学》
2017年第11期629-632,共4页
Chinese Journal of Disaster Medicine
关键词
墓头回
齐墩果酸
熊果酸
高效液相色谱法
含量测定
P. heterophylla
oleanolic acid
ursolic acid
high performance liquid chromatography
content determination