常温下厌氧氨氧化污泥的储存及活性恢复

Storage and Reactivation of Anaerobic Ammonium Oxidation(ANAMMOX)Sludge at Room Temperature

An AOB的缓慢生长严重限制了它的广泛应用,因此厌氧氨氧化污泥的长期储存和快速恢复的研究成为必然.本研究在室温(室温14~30℃)下,分别选择了15、30、45、60、75和100 d的储存时间,研究了无外加基质条件下,储存时间对厌氧氨氧化污泥活性以及恢复后活性的影响.结果显示,经过15、30、45、60、75和100 d的储存,污泥的比厌氧氨氧化活性(SAA)分别是储存前初始SAA的90.9%、64.3%、61.7%、43.2%、25.8%和19.3%.污泥的SAA随储存时间呈线性下降(R2为0.978).污泥恢复后的活性均高于污泥的初始SAA,分别为储存前的初始SAA的103.4%、129.3%、124.8%、111.7%和116.9%,储存了100 d的污泥恢复后的SAA虽未恢复到初始SAA水平,但也达到初始SAA的98.9%.试验结果表明,在长达100 d的常温储存后,厌氧氨氧化污泥的SAA(以N/VSS计)降为0.0513 g·(g·d)^(-1),经过9.5 d的恢复培养,污泥SAA仍能得到基本恢复.

Slow growth rates limit the widespread application of anaerobic ammonia oxidation（ANAMMOX） reactions,which presents a need for the study of long-term storage and rapid reactivation of ANAMMOX sludge.Under room temperature conditions（14 ～30℃）,this study investigated the effects of storage duration on the residual activity and the activity after reactivation of ANAMMOX sludge without the addition of external substances.The chosen storage durations were 15,30,45,60,75 and 100 days,respectively.The results show that the residual specific ANAMMOX activities（SAAs） of the stored ANAMMOX sludge were 90.9%,64.3%,61.7%,43.2%,25.8% and 19.3% of the initial activity（activity before sludge storage） after 15,30,45,60,75 and 100 days,respectively.Therefore,the residual SAA decreases linearly with an increase in the storage duration of ANAMMOX sludge（R～2 was 0.978）.The SAAs after reactivation with a storage duration of 15,30,45,60 and 75 days were all higher than the initial SAA and reached 103.4%,129.3%,124.8%,111.7% and 116.9% of the initial SAA before its storage,respectively.However,the SAA after reactivation after storage for 100 days was just 98.9% of the initial SAA.In conclusion,after as long as 100 days of storage duration under room temperature,the residual SAA of ANAMMOX sludge decreases to 0.051 3 g·（g·d）^-1,and its ANAMMOX activity can still be recovered after 9.5 days of reactivation culture.