腰突颗粒调节腰椎间盘退变模型兔Fas/FasL基因的表达

Yaotu Granules regulate the Fas/FasL expression in a rabbit model of lumbar disc degeneration

背景:前期研究发现,腰突颗粒可以通过延缓人髓核细胞的退变,起到保护人髓核细胞的作用。临床发现,其治疗腰椎间盘突出疗效满意。目的:探讨经验方腰突颗粒对兔腰椎间盘退变模型Fas/Fas L基因表达的影响,进一步阐明腰突颗粒防治腰椎间盘退变的作用机制。方法:将20只新西兰大白兔采用微创针刺旋切法制备兔腰椎间盘退变模型成功后,随机分为生理盐水组、低剂量组、中剂量组、高剂量组(n=5)。生理盐水组予以10 m L生理盐水灌胃,低剂量组、中剂量组、高剂量组分别予以腰突颗粒水煎剂10,20,40 m L灌胃,灌胃次数为2次/d,持续灌胃21 d。所有新西兰大白兔在相同条件下饲养,灌胃干预完成后,对各组大白兔腰椎髓核中的Fas/Fas L基因表达水平进行检测。结果与结论:(1)新西兰大白兔经微创针刺旋切法造模12周后行MRI检查证实椎间盘信号强度减弱,并见纤维环断裂,椎间盘向后突;(2)Masson染色可见纤维环细胞排列紊乱,甚至断裂,番红O染色见髓核细胞明显减少;(3)与生理盐水组相比,低剂量组、中剂量组、高剂量组椎间盘组织的Fas m RNA、Fasl m RNA表达均降低,且随着灌服腰突颗粒剂量的增加,Fas m RNA、Fasl m RNA相对表达量逐渐降低(P<0.05);(4)结果说明,腰突颗粒可降低兔腰椎兔腰椎间盘退变模型中髓核Fas/Fas L基因表达,进而延缓兔腰椎间盘退变。

BACKGROUND： Yaotu Granules have been proved to protect human nucleus pulposus cells and delay theirdegeneration. Notably, Yaotu Granules for lumbar disc herniation has achieved good clinical results.OBJECTIVE： To investigate the effect of the herbal compound formula Yaotu Granules on the Fas/FasL expression in arabbit model of lumbar disc degeneration, and further elucidate the underling mechanism of preventing and treatinglumbar disc degeneration.METHODS： Twenty New Zealand white rabbits were enrolled and the models of lumbar disc degeneration wereestablished by minimally invasive puncture and rotation cutting, followed by randomized into normal saline, low-, middle-,and high-dose groups （n=5 per group）. 10 mL of normal saline, 10, 20, and 40 mL of water decoction of Yaotu Granuleswere administered intragastrically into the normal saline, low-, middle-, and high-dose drug groups for 21 days, twicedaily, respectively. Subsequently, the expression level of Fas/FasL in the rabbit nucleus pulposus cells in each groupwas detected.RESULTS AND CONCLUSION： The signal intensity of the rabbit lumbar disc on MRI was decreased, and rupturedannulus and posterior herniated disc were visible at 12 weeks after modeling. Masson staining showed that the nucleuspulposus cells arranged in disorder, and even ruptured. Additionally, safranin O staining found that the number ofnucleus pulposus cells was decreased obviously. The order of the relative expression levels of Fas and FasL mRNA inthe nucleus pulposus cells was as follows： normal saline group 〉 low-dose drug group 〉 middle-dose drug group 〉high-dose drug group （P 〈 0.05）. These results suggest that Yaotu Granules delay the rabbit lumbar disc degenerationby downregulating the expression level of Fas/FasL.