瘢痕成熟过程中血管生成素1表达与瘢痕血管的变化

Correlation of angiopoietin-1 with angiogenesis during scar formation

背景:目前关于血管内皮细胞生长因子与瘢痕的研究较多,而血管生成素1与瘢痕的研究少有报道。目的:分析瘢痕成熟过程中血管生成素1的表达与瘢痕血管变化的关系。方法:取雌雄不限的新西兰大白兔,在耳腹侧中部相同位置制造瘢痕模型,分别在上皮化后1,2,4,8,12周切取兔耳瘢痕组织标本及兔耳腹侧正常皮肤组织,采用苏木精-伊红染色观察瘢痕成熟过程中的大体形态,CD34免疫组织化学染色观察瘢痕血管变化,Western-blot检测血管生成素1表达。结果与结论:(1)瘢痕中血管生成素1表达的趋势是先升高,至上皮化后2周最高,之后逐渐降低,至上皮化后12周最小,接近正常皮肤血管生成素1的表达;(2)微血管总数在上皮化后4周最高,以后逐渐降低;(3)成熟血管数呈逐渐升高的趋势;(4)成熟血管数/微血管总数比值呈逐渐升高的趋势;(5)兔耳瘢痕萎缩成熟过程中血管生成素1表达与成熟血管数呈负相关(P<0.05),血管生成素1表达与成熟血管数/微血管总数比值呈负相关(P<0.05);(6)血管生成素1在瘢痕萎缩成熟过程中对瘢痕血管成熟可能起重要作用。

BACKGROUND： There are many studies on the correlation between vascular endothelial growth factor and scar,but the correlation between angiopoietin-1 and scar is rarely reported.OBJECTIVE： To explore the expression of angiopoietin-1 and its correlation with angiogenesis during scar formation.METHODS： New Zealand white rabbits irrespective of gender were enrolled, and the scar models were established atthe ear ventral center. The scar tissue and normal ear ventral tissue were removed at 1, 2, 4, 8, and 12 weeks afterepithelialization. The morphology of scar formation was observed by hematoxylin-eosin staining, changes ofangiogenesis during scar formation were observed through immunohistochemical staining of CD34, and the expressionlevel of angiopoietin-1 was detected by western blot assay.RESULTS AND CONCLUSION： The expression level of angiopoietin-1 in the scar tissue was increased firstly, peakedat 2 weeks after epithelialization, then decreased gradually, and the lowest at 12 weeks close to the normal value. Thenumber of microvessels was the highest at 4 weeks after epithelialization and then decreased gradually. The number ofmature vessels was on a rise. Number of mature vessels/total number of microvessels tended to be increased. Theexpression of angiopoietin-1 was correlated negatively with the number of mature vessels, and number of maturevessels/total number of microvessels during scar formation （P 〈 0.05）. To conclude, angiopoietin-1 may play animportant role in angiogenesis during scar formation.