姜黄素通过抑制p38 MAPK和AKT活化改善幼鼠过敏性气道炎症

Curcumin Attenuates Allergic Airway Inflammation of Young Mice through Inhibiting the Activation of p38 MAPK and AKT

【目的】选择幼龄小鼠模拟3~12岁儿童的过敏性气道炎症,探索膳食补充姜黄素对幼龄小鼠过敏性气道炎症的防治作用。【方法】4周幼龄雌性BALB/c小鼠随机分为3组(每组n=8),分别是空白对照组,过敏性气道炎症模型组,姜黄素干预组。在末次卵清白蛋白激发24 h后,观察各组小鼠的症状,测定肺泡灌洗液(BALF)中的炎症细胞数,全血细胞分析白细胞亚型的数量,用HE染色观察肺部支气管周围炎症细胞浸润情况,用过碘酸-雪夫染色观察杯状细胞增生情况,酶联免疫吸附法测定BALF中的细胞因子IL-4、IL-5、IL-13以及血浆中的总Ig E水平,western blot技术检测肺组织磷酸化p38丝裂原活化蛋白激酶(p-p38 MAPK)/p38 MAPK,,p-AKT/AKT蛋白表达情况。【结果】与过敏性气道炎症模型组相比,饲料添加姜黄素的干预组可以明显改善幼鼠的反复抓挠鼻子、点头呼吸、体质量减轻等症状,外周血中嗜酸性粒细胞降低具有统计学差异(P<0.05),肺泡灌洗液和肺支气管周围的炎症细胞以及肺泡灌洗液中IL-4,IL-5,IL-13的水平降低(P<0.05),支气管上皮杯状细胞增生减轻,此外,肺组织的磷酸化p38 MAPK和磷酸化AKT水平降低,组间差异有统计学意义(P<0.05)。【结论】膳食添加姜黄素可以抑制幼龄小鼠过敏性气道炎症,其机制可能与抑制肺组织的p-38 MAPK和AKT信号传导有关。

【Objective】To investigate the effects of dietary curcumin on the prevention and treatment of allergic airway inflammation in young mice for simulating the allergic airway inflammation of 3-12 years old children.【Methods】The 4 weeks young female BALB/c mice were randomly divided into three groups(n=8): Control group, Model group and curcumin group(800 mg curcumin/kg diet). 24 h after the last OVA challenge, the symptoms of mice in each group were observed, the inflammatory cells in the bronchoalveolar lavage fluid(BALF) were measured, various kinds of blood cells were detected, the inflammatory cells around the peribronchial areas stained by H&E and the goblet cell hyperplasia in the lungs stained by PAS were analyzed. Additionally, the IL-4, IL-5, IL-13 in the BALF and the total Ig E level in the plasma were detected by ELISA, and the activation of p38 MAPK and AKT was measured by western blot. 【Results】 The mice of model group showed the symptoms of allergic airway inflammation, such as repeatedly scratched the noses, showed nodding breath, notably, the weight of model mice was decreased significantly during the OVA challenge phase, while the symptoms mentioned above were alleviated in curcurmin group. The blood cells test found that the curcumin could inhibit the elevation of the eosinophils significantly(P<0.05). Dietary curcumin treatment significantly decreased the inflammatory cells in the BALF and peribronchial areas, and the IL-4, IL-5, IL-13 levels in the BALF were significantly inhibited(P<0.05). The goblet cell hyperplasia was attenuated by curcumin treatment, and the dietary curcumin inhibited the activation of p38 MAPK and AKT signaling.【Conclusions】Dietary curcumin can alleviate the allergic airway inflammation of young mice, which may through inhibiting the transduction of p-38 MAPK and AKT signaling.