基于氨基化碳量子点荧光猝灭法检测苏丹Ⅳ的研究

Determination of sudan Ⅳ based on fluorescence quenching of amino-functional carbon quantum dots

通过一步水热法以葡萄糖为原料合成稳定性高的碳量子点,并用氨水使其表面氨基化。苏丹Ⅳ能使氨基化的碳量子点荧光明显猝灭,由此建立一种简便、高效检测苏丹Ⅳ的新方法。考察了缓冲体系pH、反应时间、反应温度等对苏丹Ⅳ测定的影响。结果表明,在pH=8.4的硼酸-硼砂缓冲溶液中,室温反应30min时,体系的F0/F与苏丹Ⅳ浓度呈良好的线性关系,其线性范围为2.0×10-6 mol·L^(-1)~9.0×10-5 mol·L^(-1),相关系数R^2=0.9913,检出限为6.4×10^(-7)mol·L^(-1)。方法用于食品中苏丹Ⅳ含量的检测,加标回收率为90.0%~102.5%。

With crystal giucose as raw material, the carbon quantum dots （ CQDs） and amino-functional carbon quantum dots（N- CQDs）were synthesized through one-step hydrothermal method. Based on the significant quenching effect of Sudan IV on the fluo-rescence of N-CQDs,a method with high sensitivity and good selectivity for the determination of Sudan IV was established Different influencing factors were studied including pH buffer system, reaction time, reation temperature and so on. The results show that, in borate-borax buffer solution of 8. 4,the reaction of room temperature 30 min,the ratio of fluorescence intensities（F 0/ F） and Sudan IV concentration had good linear relationship,the linear range is 2. 0×10 6mol · L ︿-1 -9. 0 ×10︿-5 mol · L︿-1, the correlation coeffi-cient R2 =0. 9913 ,the detection limit is 6. 4× 10 7mol · L ︿-1. The method is simple and applicable to the determination of Sudan IV in food samples with satisfactory results,the recoveries were 90. 0%to 102. 5%.