青天葵组织培养球茎诱导的研究

Study on Tissue Culture Corms Inducing of Nervilia fordii

【目的】研究蔗糖、大量元素和激素等浓度因素对青天葵(Nerviliae fordii)组培球茎诱导形成的影响。【方法】以青天葵球茎诱导形成的走茎,并切取生长端长度约为3 cm带节的茎段为试验材料。分别在以MS培养基为基本培养基,含不同蔗糖、KNO_3、NH_4NO_3、KH_2PO_4、6-BA、NAA和PP333浓度的培养基中培养,统计形成球茎的数量、直径和鲜重等指标。【结果】蔗糖浓度为40~50 g/L时球茎诱导效果较好;MS培养基中的大量元素,当KNO_3、NH_4NO_3、KH_2PO_4浓度分别为2800~2600、1650~1980和230~272 mg/L时诱导效果较好。在MS培养基中附加终浓度为0.5~2.5 mg/L NAA和0.4~0.6 mg/L PP333时,提高了青天葵组培球茎的形成速度且形成的球茎大小均匀;附加6-BA则不利于球茎诱导。【结论】筛选出的培养基和培养条件可有效提高青天葵球茎诱导效率。

【Objective 】To explore the influence of concentration factors of sucrose, macroelements and hormones on the induction of tissue cultured corms, the tissue cultured amphitropous stem of was selected as explants and studied.【 Methods】 The amphitropousstem induced by Nerviliae fordii corms were cut at about 3 cm of growing end as experimental materials, and cultured in MS medium with dif-ferent concentration of sucrose, KN03 , NH4N03 , KH2P〇 4 , 6 -BA, NAA and PP333 respectively. [ Result]Sucrose at the concentration of 40 - 50 g/L performed better induction effect. KN03 performed better induction effect at the concentration of 2800 - 2600 mg/L. NH4N03 had better induction effect at the concentration of 1650 - 1980 mg/L. KH2P〇 4 performed better induction effect at the concentration of 230 -270 mg/L. MS medium supplemented with 0. 5 - 2. 5 mg/L NAA and 0. 4 - 0. 6 mg/L PP333 accelerated the formation of Nerviliae fordii tissue cultured corms, with no influence on the quantity and uniformity of size. 6 -BA was not conductive to the induction of tissue cultured corms.【 Conclusion】 The selected medium and culture conditions can increase the induction efficiency of iVerz^aecorms.