希美宁对T2DM模型大鼠血清中GLP-1、GIP含量及骨骼肌PI3KmRNA、AktmRNA表达的影响

Effect of Ximeining on the Levels of GLP-1 and GIP in Serum and the Expression of PI3KmRNA and AktmRNA in Skeletal Muscle of Type 2Diabetic Rat

目的:观察希美宁对2型糖尿病(T2DM)模型大鼠血清中胰高血糖素样肽-1(GLP-1)、葡萄糖依赖性促胰岛素释放肽(GIP)含量及骨骼肌中磷脂酰肌醇-3-激酶(PI3KmRNA)、丝苏氨酸蛋白激酶(AktmRNA)表达的影响。方法:脂肪乳联合2次小剂量注射链脲佐菌素(STZ)复制2型糖尿病大鼠模型,将大鼠随机分为空白组、模型组、希美宁组、二甲双胍对照组,酶联免疫法(ELISA)检测各组大鼠血清中GLP-1、GIP的含量,RT-PCR法检测各组大鼠骨骼肌中PI3KmRNA、AktmRNA的表达水平。结果:与模型组相比,希美宁组、二甲双胍对照组可增加大鼠血清中GLP-1、GIP的含量(P<0.05或P<0.01),亦可显著上调骨骼肌中PI3KmRNA、AktmRNA的表达(P<0.01)。结论:希美宁可通过促进GLP-1、GIP的分泌来调节机体代谢,亦可通过改变PI3KmRNA、AktmRNA的表达改善T2DM模型大鼠的胰岛素抵抗。

Objective：To observe the effects of Ximeining on the levels of GLP-1 and GIP in serum and the expression of PI3KmRNA and AktmRNA in skeletal muscle of type 2 diabetic rats.Methods：Intragastric administration of fat emulsion and two small dose injection of STZ induced type 2 diabetic rat model,the rats were randomly divided into controlled group,model group,Ximeining group,metformin group,enzyme linked immunosorbent assay was used to detect the levels of GLP-1 and GIP in serum of rats in each group.The expression of PI3KmRNA and AktmRNA in skeletal muscle of rats were detected by RT-PCR method.Results：Compared with the model group,Ximeining group and metformin group can increase the content of serum GLP-1,GIP（P（0.05 or P（0.01）,also can significantly up regulate the expression of PI3KmRNA and AktmRNA in skeletal muscle（P（0.01）.Conclusion：Ximeining can promote GLP-1 and GIP secretion to regulate metabolism,but also by the change of expression of PI3KmRNA and AktmRNA to improve the T2DM rat model of insulin resistance.