桂野桐中香豆素类化学成分的研究

Coumarins from Mallotus conspurcatus

目的研究桂野桐中的香豆素类化学成分。方法利用硅胶、葡聚糖凝胶、MCI填料(Middle chromatogram isolated Gel)、半制备HPLC等色谱分离手段对桂野桐体积分数75%乙醇粗提物的乙酸乙酯萃取物进行分离,运用~1H-NMR、^(13)C-NMR、ESI-MS等现代谱学方法以及与文献数据对比,鉴定化合物的结构。结果分离得到12个香豆素,它们分别是malloapelin A(1)、5'-去甲氧基沉香木质素(2)、沉香木质素(3)、cleomiscosin B(4)、6-羟基-7-甲氧基-香豆素(5)、braylin(6)、aegelinol(7)、佛手内酯(8)、7-羟基-6,8-二甲氧基香豆素(9)、8-羟基-6,7-二甲氧基香豆素(10)、银桦内酯(11)和秦皮素(12)。结论化合物6~12为首次从该属植物中分离得到。

OBJECTIVE To study the coumarins from Mallotus conspurcatus. METHODS The ethyl acetate part of 75% etha- nol extracts from Mallotus conspurcatus was isolated. The compounds were isolated and purified with column chromatography, and their structures were elucidated on the basis of 1H-NMR, 13 C-NMR and ESI-MS dat^u RESULTS Twelve coumarins were determined to he malloapelin A （ 1 ）, 5＇-demethyl aquillochin （ 2 ）, aquillochin （3）, eleomiseosin B （4）, 6-hydroxy-7-methoxy-coumarin （5）, braylin （6）, aegelinol （7）, bergapten （8）, isofraxidin （9）, fraxidin 10）, grevillone （11）, and fraxetin （12）. CONCLUSION Com- pounds 6 - 12 are isolated from this genus for the first time.