基于变异系数权重的模糊物元模型评价当归不同提取部位补血的效果

Evaluating the effect of blood nourishing of Angelica's different extracted parts with Fuzzy Matter-element model based on variation coefficient weight

目的筛选当归补血的有效部位并探讨模糊物元模型在当归不同提取部位补血效果综合评价中的应用。方法按照体重将昆明随机分为10组:正常组,模型组,阳性对照组(复方阿胶浆),实验组(部位1-7),每组10只。用环磷酰胺(CTX)与乙酰苯肼(APH)合用建立小鼠血虚模型。以外周血常规、胸腺(TI)及脾指数(SI)为检测指标,观察当归不同提取部位的补血效果;用基于变异系数权重的模糊物元模型,对补血的效果进行综合评价。结果与模型组的TI、SI、红细胞(RBC)计数、血小板(PLT)计数、红细胞压积(HCT)、血红蛋白(HGB)含量、白细胞(WBC)计数分别为(7.70±1.81)×10^(-2),(56.90±12.23)×10^(-2),8.44±1.59,1453.10±102.50,56.54±6.69,170.00±26.39,2.10±0.49相比较,70%乙醇浸渍部位(部位2)的TI,SI,RBC,PLT,HCT,HGB,WBC分别为0.10±2.66×10^(-2),(33.83±8.97)×10^(-2),11.94±2.07,1749.60±211.50,55.53±9.42,55.53±9.42,2.60±0.47,部位2小鼠的这7个指标减小,其中SI、RBC、PLT、HCT、WBC差异均有统计学意义(P<0.05或P<0.001)。部位1和部位2相比较,各项指标差异均无统计学意义(P>0.05)。在部位1-7中,反映补血总效应的最大贴进度值为0.50(部位2),大于0.40(部位1)。结论 70%乙醇浸渍部位为当归补血的有效部位;基于变异系数权重的模糊物元模型可客观、准确的评价当归不同提取部位补血的综合效果。

Objective To select the effective parts of blood nourishing and to investigate the utilization of fuzzy matter-element model in evaluating the effect of blood nourishing of Angelica＇s different extracted parts. Methods KM mice half males and half females were randomly divided into 10 groups, including blank control group, model group（ blood-deficiency）,positive control group（ Complex Prescription Glue Mucilage： 0. 02 mL· g^-1）,experimental group consisted by 7 different extracted parts of Angelica（ 3. 4 g · kg^-1 Angelica herbs）.Acetylphenyhydrazine（ APH） and cyclophosphamide（ CTX） were used to copy the blood-deficiency model mice by administration APH at the second day（ 20 mg · kg^-1） and the fifth day（ 40 mg · kg^-1）respectively,since the fifth day,ip CTX 40 mg·kg^-1 everyday,once a day for four days. Periphera hemogram and organ index,including thymus index（ TI）,spleen index（ SI）,were detected at the 10 th day. Then usingFuzzy Matter-element model on variation coefficient weight. Results The TI,SI,red blood cell（ RBC）,platelet count（ PLT）,hematokrit（ HCT）,hemoglobin（ HGB）,white blood cell（ WBC） were（ 7. 70 ± 1. 81） ×10^-2,（ 56. 90 ± 12. 23） ×10^-2,8. 44 ± 1. 59,1453. 10 ± 102. 50,56. 54 ± 6. 69,170. 00 ± 26. 39,2. 10 ± 0. 49,respectively in model group. The 70% ethanol impregnation part（ part 2） for TI,SI,RBC,PLT,HCT,HGB,WBC were0. 10 ± 2. 66 ×10^-2,（ 33. 83 ± 8. 97） ×10^-2,11. 94 ± 2. 07,1749. 60 ± 211. 50,55. 53 ± 9. 42,55. 53 ± 9. 42,2. 60 ± 0. 47,respectively. The part 2 can increase TI,RBC,PLT,HCT,WBC and reduce SI,HGB. Compared with model group,the difference was statistically significant in SI,RBC,PLT,HCT,WBC（ P〈0. 05 or P〈0. 001）. There were no significant differences between part 1 and part 2（ P〈0. 05）. Combining with the result of the value of Euclid approach degree,in part 1-7,the maximum is 0. 50（ part 2）,is greater than 0. 40（ part 1）. Conclusion The 70%alcohol impregnation part is the effective part of blood nourishing. The method and model can be used to objectively evaluate the comprehensive effect of blood nourishing of Angelica＇s different extracted parts.