二苯乙烯苷、远志皂苷元2∶1配伍抗Aβ_(25-35)损伤PC12细胞作用的最适浓度研究

Optimal concentrations of stilbene glucoside andsenegenin combined at a ratio of 2:1 in treatment of PC12 cell injury induced by Aβ_(25-35)

目的:研究不同浓度下二苯乙烯苷(TSG)、远志皂苷元(TEN)2∶1配伍抗Aβ25-35损伤PC12细胞的作用,以筛选最适配伍浓度。方法:采用MTT比色法筛选Aβ25-35最适造模浓度,在所得最适Aβ25-35浓度基础上采用MTT比色法筛选TSG-TEN合用抗Aβ25-35损伤PC12细胞作用的最适浓度。结果:与正常组比较,模型组的细胞存活率明显降低(P<0.01);与模型组比较,合用4组(100μmol/L TSG+50μmol/L TEN)的细胞存活率最高(P<0.01)。结论:在本实验所选取的浓度范围内,TSG与TEN 2∶1配伍抗Aβ25-35损伤PC12细胞以"100μmol/L TSG+50μmol/L TEN"合用浓度为最佳。

Objective ：To investigate the effect of different concentrations of stilbene glucoside （TSG）and senegenin（TEN） combined at a ratio of 2：1 on PC12 cell injury induced by Aβ25-35, and to screen out the optimal concentrations for combination. Methods：MTT colorimetry was used to screen out the optimal concentration of Aβ25-35 for modeling, and then it was used to screen out the optimal concentrations of TSG -TEN combination in the treatment of PC12 cell injury induced by Aβ25-35. Results：Compared with the normal group,the model group had a significant reduction in the survival rate of cells （P 〈 0. 01 ） ; compared with the model group, the combination group 4 （ 100 μmol/L TSG ＋ 50 μmol/L TEN） had the highest survival rate of cells （P 〈 0. 01 ）. Conclusion：Within the range of concentrations selected in this study, 100 μmol/L TSG ＋ 50 μmol/L TEN is the optimal concentrations for the combination of TSG and TEN at a ratio of 2：1 in the treatment of PC12 cell injury induced by Aβ25-35.