刺五加与短梗五加中紫丁香苷、刺五加苷E及异嗪皮啶含量比较

Comparison of Eleutheroside B, Eleutheroside E, and Isofraxidin between Acanthopanax sentcosus(Ruper.et Maxim.) Harms and Acanthopanax sessiliflorus

为比较刺五加及短梗五加样品中主要活性成分差异,选取吉林、黑龙江及辽宁等三省刺五加及短梗五加样品,利用薄层层析(TLC)法进行鉴别,并利用HPLC法测定了紫丁香苷、刺五加苷E及异嗪皮啶含量。结果表明:利用紫外灯(365 nm)显示的刺五加TLC图谱中异嗪皮啶特征条带均非常明显,短梗五加TLC图谱中异嗪皮啶特征条带不明显但存在痕迹,因此该鉴别方法可增加定量指标以保证鉴别结果的确定性。HPLC测试结果显示刺五加样品中三种功效成分含量总体较高,且紫丁香苷及异嗪皮啶平均含量均高于短梗五加样品。个别短梗五加中紫丁香苷含量达到近0.1%,而《药典》中规定紫丁香苷含量不低于0.05%,因此为防止短梗五加的混入,可在进一步深入研究基础上适当提高刺五加中紫丁香苷含量标准。

Thin Layer Chromatography（TLC） was used to identify the differences of main active components between Acanthopanax sentcosus（Ruper.et Maxim.） Harms（ASR） and Acanthopanax sessiliflorus（AS）, which were obtained from Jilin, Heilongjiang and Liaoning. The content of Eleutheroside B, Eleutheroside E and Isofraxidin were determined by High Performance Liquid Chromatography（HPLC）. The results showed that the Isofraxidin characteristic band of ASR displayed on TLC chromatogram was obvious using a ultraviolet lamp（365 nm）. While the Isofraxidin characteristic band of AS was not obvious, it still existed on TLC chromatogram. Consequently, the identification method could improve quantitative indicator that was to give a definite conclusion. The HPLC results showed that the content of three functional components in ASR samples were high, and the average content of Eleutheroside B and Isofraxidin of ASR samples were higher than that of AS samples. The content of Eleutheroside B of several AS samples was nearly 0.1%, which was higher than the level regulated by Chinese Pharmacopoeia（0.05%）. The content standard of Eleutheroside B could be improved for further study to avoid the adulterant of ASR.