一种简便的环糊精超分子包结比测算新方法

A new and simple method for calculating the inclusion ratio of cyclodextrin supramolecules

利用紫外可见分光光度计,通过测定β-环糊精-山梨酸超分子包合物的紫外吸光度,提出一种新的差量法用以测算该超分子的包结比,其值与经典的摩尔比法及相溶解度法相同,均为11,表明该类超分子是由1分子主体分子(β-环糊精)包结1分子客体分子(山梨酸)而成。差量法为多次测量吸光度再取平均值进行测算,其结果较摩尔比法更为准确;其测算过程,不受外界因素干扰,无需绘制相图,较相溶解度法更为简便。

Based on UV absorbance,a new and simple subtraction method was proposed for the calculation of the inclusion ratio,and it was proved to be equal in number to the classical molar ratio or phase solubility method.Using any kind of these three methods,the results always showed that the inclusion ratio of this complex was 11,which indicated that this kind of supramolecular complex was formed through an inclusion interaction between 1 host molecule（β-cyclodextrin）and 1 guest molecule（sorbic acid）.Sincethe subtraction methodadopted a repetitive measurement and took the averaged,its result was more accurate than the molar ratio method;meanwhile,due to the advantage of less interference factors and especiallyno need of drawing a complicated phase diagram,its calculation process was much simpler than the phase solubility method.