摘要
目的通过免疫组织化学方法观察小鼠牙本质生成过程中Osterix(Osx)的表达变化,探索其功能及意义。方法取小鼠不同年龄段及建立修复性牙本质模型的下颌骨,石蜡包埋切片后分别进行HE染色和免疫组织化学染色。结果在钟状期晚期,Osx在牙尖处成牙本质细胞核内表达。小鼠出生后3 d,Osx在牙冠的全部成牙本质细胞核内高表达。2周龄小鼠,冠部成牙本质细胞核内几乎不表达Osx,磨牙根部成牙本质细胞核内Osx高表达。牙尖磨损建模7 d后,Osx在成牙本质细胞核内表达升高,14 d时表达量下降。结论 Osx在促进成牙本质生成和修复过程中发挥作用。
Objective To explore the expression of Osterix during detinogenesis by Immunohistochemistry（ IHC） staining and its function and meaning. Methods The mandibles of the mice at different age stages were taken as specimens to establish a model by HE and IHC staining after processing. Results The results of immunohistochemistry showed that Osx didn＇t expressed in the nucleus of odontoblast until the late bell stage. All odontoblasts under the crown highly expressed Osx on day 3. While IHC staining revealed that Osx rarely expressed in odontoblasts in the crown on day 14,but highly expressed in the root. Osx highly expressed in odontoblasts after 7 days of operation,while was down regulated after 14 days. Conclusion It can be concluded that Osx protein may have a role during dentinogenesis and participate in the repairation of odontoblast.
作者
李功臣
胡凌玮
许婷婷
孙瑶
LI Gongchen;HU Lingwei;XU Tingting;SUN Yao.(Department of lmplantology, School ＆ Hospital of Stomatology, Tongfi University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, Chin)
出处
《口腔医学》
CAS
2017年第11期965-968,共4页
Stomatology
基金
国家自然基金(81300840
81470715)
2013年教育部博士点基金(20130072120024)
上海市卫生系统新优青项目(XYQ2013080)
国家重点研发计划(2016YFC1102705)
