基于UFLC-Q-TOF-MS/MS技术及PCA分析的不同产地杏香兔耳风药材差异研究

Study on differences of Ainsliaea fragrans from different sources based on UFLC-Q-TOF-MS/MS and PCA analysis

该研究建立UFLC-Q-TOF-MS/MS技术结合多元统计分析法快速、简便、准确鉴别不同产地杏香兔耳风药材差异性的方法。采用超高速液相-飞行时间串联质谱仪(UFLC-Q-TOF-MS/MS)对江西、云南、江苏和河南地区等产地的杏香兔耳风药材进行检测,质谱采用ESI源的负离子模式,利用Markerview等软件对数据结果进行PCA和OPLS-DA聚类分析及t检验。结果显示在负离子模式下不同产地的药材主成分得到了良好的区分,聚类结果同时体现了杏香兔耳风药材在地域间的差异性和相似性,共鉴定出40种差异成分。该研究建立的鉴别杏香兔耳风药材产地差异性的方法具有快速准确和简便等优势,为杏香兔耳风不同产地的药材质量评价奠定了基础。

A rapid and accurate method of UFLC-Q-TOF-MS/MS combined with multivariate statistical analysis was established for the identification of Ainsliaeafragrans from different origins in this study. The A. fragrans from different producing areas of Jiangxi, Yunnan, Henan and Jiangsu were determined by UFLC-Q-TOF-MS/MS in the negative ion mode. And the data of the study were ana- lyzed by the Markerview and other software for the PCA and OPLS-DA cluster analysis as well as t test. The results of the principal component analysis（PCA） showed that the main components from different origins were well distinguished. And the results of multivari- ate statistical showed the differences and similarities between different producing areas. Besides, 40 different compounds were identi- fied in the negative ion mode. This method for identifying A. fragrans from different producing areas has the advantages of rapid accura- cy and simplicity, which laid the foundation for the evaluation of the quality of the A. fragrans.