微小RNA-340对肝癌细胞增殖和凋亡的影响

Effect of MicroRNA-340 on Proliferation and Apoptosis of Hepatocellular Carcinoma Cell Lines

目的探讨过表达mi R-340对人肝癌Hep G2、MHCC-97L细胞增殖、细胞周期和凋亡的影响。方法 mi R-340 mimic转染后RT-PCR法测定肝癌细胞株mi R-340 m RNA水平,Brdu-ELISA试剂盒检测细胞增殖,流式细胞术分析细胞周期,膜联蛋白V-FITC凋亡检测试剂盒分析细胞凋亡。结果mi R-340在肝癌细胞系中表达降低(P<0.05);高表达mi R-340后肝癌细胞的存活率受到显著抑制(P<0.05);G0/G1期细胞增多(P<0.05);S期细胞减少(P<0.05);高表达mi R-340后Hep G2和MHCC-97L细胞凋亡受到促进(P<0.05)。结论 mi R-340在抑制细胞增殖、NF-κB1下调诱导的细胞凋亡中起着重要的作用,提示mi R-340表达降低是肝细胞肝癌发生的机制之一。

Objective To explore the effect of mi R-340 overexpression on cell proliferation, cell cycle and apoptosis of human hepatocellular carcinoma Hep G2 and MHCC-97 L cells. Methods The mi R-340 mimic was transiently transfected into HCC cells using Lipofectamine？ 2000 reagent. The m RNA level of mi R-340 in HCC cell lines was determined by RT-PCR. Cell proliferation was detected by Brdu-ELISA kit. Flow cytometry was used to analyze cell cycle. Apoptosis was analyzed by Annexin V-FITC apoptosis detection kit. Results The expression of mi R-340 was decreased in Hep G2 and MHCC-97 L cells（P〈0.05）; mi R-340 overexpression signifi cantly inhibited cell survival rate（P〈0.05）; the percentage of cells in G0/G1 stage was increased and that in the S stage was reduced（both P〈0.05）; mi R-340 overexpression promoted the apoptosis of Hep G2 and MHCC-97 L cells also. Conclusion mi R-340 plays an important role in the inhibition of cell proliferation and the apoptosis induced by NF-κB1 down-regulation, suggesting that reduced mi R-340 expression is one of the mechanisms of hepatocellular carcinogenesis.