血必净注射液治疗火器致兔ALI的康复价值及对肺组织NF-κB表达的影响

The value of Xuebijing Injection on the rehabilitation of rabbits with acute lung injury induced by firearm injury and the effect on the expression of NF-κB in lung tissue

目的探讨血必净(XBJ)注射液治疗火器致兔急性肺损伤(ALI)的康复价值及对肺组织核因子-κB(NF-κB)表达的影响。方法采用随机数字表法将SPF级健康雄性兔60只分为正常对照组、ALI模型组、生理盐水组、低剂量XBJ治疗组、中剂量XBJ治疗组、高剂量XBJ治疗组,每组各10只。ALI模型组、生理盐水组、低剂量XBJ治疗组、中剂量XBJ治疗组、高剂量XBJ治疗组吸入高温气体,制作完成兔ALI动物模型;建模半小时后,低、中、高剂量XBJ治疗组分别腹腔内注射10、20、30 mg/kg XBJ,生理盐水组注射生理盐水,正常对照组和ALI模型组不做处理。观察12 h后,处死兔并取左肺上叶肺组织,检测肺组织NF-κB的表达;在光镜下对肺组织进行病理学检查,并及时记录其病理变化。结果病理学检查结果:正常对照组肺泡结构清晰,肺泡壁薄,肺泡内未见水肿液;ALI模型组、生理盐水组肺泡结构模糊,肺泡壁断裂,肺内大量炎症细胞浸润,肺组织间质及肺泡腔出现明显水肿,可见较多的红细胞,肺泡壁间质增厚,透明膜形成;经XBJ治疗的各组肺泡结构仍存在,肺内炎症细胞浸润减轻,肺组织间质及肺泡腔中度水肿,可见少量渗液、出血。肺组织Western blot分析显示,ALI模型组NF-κB活性明显高于正常对照组(P<0.01);与ALI模型组、生理盐水组比较,低、中、高剂量XBJ治疗组兔肺组织中NF-κB活性明显降低,差异均有高度统计学意义(P<0.01),其中,中剂量XBJ治疗组降低最明显,与低、高剂量XBJ治疗组比较差异均有统计学意义(P<0.05、P<0.01)。结论 XBJ注射液对火器伤诱导兔的ALI具有保护作用,且XBJ中剂量保护作用最明显。

Objective To investigate the value of Xuebijing Injection （XBJ） on the rehabilitation of rabbits with acute lung injury （ALI） induced by firearm injury and the effect on the expression of nuclear factor kappa B （NF-κB） in lung tissue. Methods Sixty heahhy male rabbits were randomly divided into normal control group, ALI model group, normal saline group, low dose of XBJ treatment group, medium dose of XBJ treatment group, high dose of XBJ treatment group by random number table mehtod, with 10 rats in each group. The rabbits of ALI model group, normal saline group, low dose of XBJ treatment group, medium dose of XBJ treatment group, high dose of XBJ treatment group were inhaled with high-temperature gas, so as to complete the model of rabbits with ALI. After modeling for half an hour, low, medium, high dose of XBJ treatment groups were respectively received intraperitoneal injection of 10, 20, 30 mg/kg XBJ, normal saline group was injected with normal saline, while normal control group and ALI model group were not dealt with any treatment. After 12 h, the rabbits were killed and the lung tissue of left upper lobe was taken to detect the expression of NF-κB in lung tissue; under the light microscope, the lung tissue was taken to make pathological ex- amination, and the pathological changes were recorded in time. Results The results of pathological examination： in thenormal control group, the alveolar structure was clear, the alveolar wall was thin, and no edematous fluid was found in the lung tissue; in the ALl model group and normal saline group, the alveolar structure wasfuzzy, the alveolar wall was broken, there were a lot of inflammatory cells infiltration in the lung, obvious edema was found in the pulmonary mesenchyme and alveolar space, many red blood cells could be seen, and the mesenchyme of alveolar wall was thickened, the hyaline membrane was formed; after treatment by XBJ, the alveolar structures in all groups were still existed, the inflammatory cells infiltration in the lung was reduced, pulmonary mesenchyme and alve- olar space showed moderate edema, less cataclysm and bleeding could be seen. Western blot of lung tissue showed that, the activity of NF-κB in ALI model group was significantly higher than that of normal control group （P 〈 0.01）; compared with ALI model group and normal saline group, the activity of NF-κB in lung tissue of low, medium, high dose of XBJ treatment groups were all decreased, the differences were all highly statistically significant （P 〈 0.01）, a- mong which, medium dose of XBJ treatment group was decreased most significantly, which had statistically significant differences compared with those of low, high dose of XBJ treatment groups （P 〈 0.05, P 〈 0.01）. Conclusion XBJ in- jection has protective effects on rabbits with ALl induced by firearm injury, and the protective effect of medium dose of XBJ is the most obvious.