15-LOX-1对LPS作用下肺动脉内皮细胞增殖和炎性因子的影响

The effect of 15-LOX-1 on proliferation of pulmonary arterial endothelial cells and inflammatory cytokines by LPS

目的探讨下调15-脂氧酶-1(15-LOX-1)对脂多糖(LPS)作用下肺动脉内皮细胞(PAECs)和炎性因子分泌的影响。方法体外培养PAECs,并随机分为4组:对照组、LPS组、15-LOX-1 si RNA对照组(scramble组)和15-LOX-1 si RNA组。采用Real-time PCR检测15-LOX-1表达,MTT法检测PAECs增殖变化,ELISA法检测产物13-HODE以及炎性因子白细胞介素1α(IL-1α)和白细胞介素6(IL-6)的变化。结果与LPS组比较,15-LOX-1si RNA组15-LOX-1 m RNA表达降低,PAECs增殖增多,13-HODE、IL-1α和IL-6分泌减少,差异有统计学意义(P<0.05)。结论下调15-LOX-1可抑制炎性因子分泌,促进PAEC增殖,改善LPS对内皮的损伤。

Objective To explore the effect of 15-LOX-1 on proliferation of pulmonary arterial endothelial cells （PAECs） and inflammatory cytokines by LPS. Methods PAECs were cultured in vitro, and divided into 4 groups, including control group, LPS group, 15-LOX-1 siRNA scramble group （scramble group） and 15-LOX-1 siRNA group. The expression of 15-LOX-1 mRNA was detected by Real-time PCR. The proliferation of PAECs was examined by MTT. The expressions of 13-HODE, IL-1α and IL-6 were detected by ELISA. Results In the 15-LOX-1 siRNA group, the expression of 15-LOX-1 mRNA was reduced, the proliferation of PAECs was increased, and the secretions of 13- HODE, IL-1α and IL-6 were reduced compared with the LPS group with statistically significant difference （P 〈 0.05） Conclusion Down-regulation of 15-LOX-1 can inhibit inflammatory cytokines, regulate PAECs proliferation, and improve endothelial injury induced by LPS.