扶肾颗粒改善腹膜超滤功能的分子机制

Molecule Mechanism of Fushen Granules in Improving Peritoneal Ultrafiltration Function

目的:观察扶肾颗粒对腹膜透析大鼠的腹膜超滤功能、乙二醛酶-1(glyoxalase 1,Glo-1)及终末期糖基化产物(advanced glycation end products,AGEs)表达的影响。方法:建立5/6肾切除肾衰模型,将造模成功的的SD大鼠随机分为1.5%腹透液(peritonealdialysis solution,PDS)模型组;1.5%PDS+扶肾颗粒干预组;4.25%PDS模型组;4.25%PDS+扶肾颗粒干预组,每组17只;另设空白组,15只,4周后处死,取各组大鼠腹膜组织及血清,计算腹膜超滤量,蛋白免疫印迹法(Western blot)检测大鼠腹膜组织Glo-1蛋白表达水平,酶联免疫吸附法(ELISA)检测血清AGEs的含量。结果:扶肾颗粒干预组腹膜超滤量、血清AGEs含量比同浓度透析模型组均降低(P<0.05,P<0.01)。与空白组比较,模型组Glo-1蛋白表达增加(P<0.05);1.5%PDS模型组Glo-1蛋白表达水平虽总体高于同浓度PDS药物干预组,但无明显统计学差异;4.25%PDS模型组与同浓度PDS药物干预组比较,Glo-1蛋白表达水平显著增高(P<0.05)。结论:扶肾颗粒可以调节Glo-1的表达水平并减少血清AGEs的蓄积,从而保护残肾功能,延缓腹膜超滤衰竭及腹膜纤维化。

Objective ： To observe the effect of Fushen granules on the glyoxalase-1 （GIo-1） and advanced glycation end products （AGEs） in rats with peritoneal ultrafiltration function, peritoneal dialysis. Method ： The chronic renal failure peritoneal dialysis rat model was induced through 5/6 nephrectomy. After successful modeling, the SD rats were randomly divided into 1.5% PD model group; 1.5% PD ＋ Fushen granules intervention group; 4.25% PD model group; and 4.25% PD ＋ Fushen granules intervention group （group E）, with 17 rats in each group; the control group was also set up, with 15 rats. After 4 weeks, the rats were put to death, and their peritoneal tissues and serum were collected to calculate the peritoneal ultrafiltration volume; Western blot was used to detect the rat peritoneal organization Glo-1 protein expression. And enzyme-linked immunoassay （ELISA） was used to detect the content of serum AGEs. Result： Peritoneal ultrafihration and serum AGEs content in Fushen granules intervention group were lower than dialysis model group of the same concentration, with stati.-tically significant differences （ P 〈 0.05, P 〈 0. 01 ）. Compared with the control group, Glo-1 protein content increased in the model group, with statistically significant differences （P 〈 0.05）. Glo-1 protein expression in group B was higher than that in group C, but with on statistically significant difference. Compared with group E, Glo-1 protein expression was significantly elevated, with statistically significant differences （P 〈 0.05）. Conclusion： Fushen granules can regulate the expression of Glo-1 and reduce the accumulation of serum AGEs, so as to protect the residual kidney function and delay peritoneal filtration and peritoneal fibrosis.