摘要
2016年6月,广西邕宁某场有20%的阳江鹅在喙、眼和脚出现赘肉状痘癍,临床无死亡病例。为了研究此病的病原和基因特征,采集痘癍样本、制备病理组织切片进行显微镜观察、并利用透射电镜进行形态学分析以及TaqMan荧光定量PCR检测,证实其病原为禽痘病毒属(Avipoxvirus,APV)成员,命名为YNE。通过对病毒核芯蛋白P4b编码基因(fpv167)和DNA聚合酶Popr编码基因(fpv094)的部分序列串联进行比对,发现该毒株与1991年分离于北美林鸳鸯的APV高度同源,属于A5.2基因分支。进一步序列分析发现,该毒株与同期发生在该场的麻鸭源APV相应序列的同源性可达99.9%,初步证实该病毒可以在鸭鹅间跨种传播。
We undertook pathogen and genetic characterization of a virus that caused an unidentified disease in Yangjiang geese in Yongning County （Guangxi Province, China） in June 2016. Clinical signs were cuta- neous nodules on the eyelids, beaks, and legs. About 20% of the Yangjiang geese were affected by this mystery disease but no deaths were observed. Clinical samples were collected and underwent histopatho- logic examination, electron microscopy analysis, and Taqman real-time polymerse chain reaction. An avi- poxvirus was identified as a pathogen of this disease outbreak, and designated ＂YNE＂. Furthermore, the partial sequences of the vial P4b gene （fpv167 locus） and DNA polymerase gene （fpv094 locus） were am- plified and analyses. The YNE group was in a new cluster within subclade A5.2, which comprises avipox- virus from an outbreak in wood duck in the USA in 1991. Nucleotide identity of 99.9% was also found be- tween YNE and YNY （which was isolated from mallard duck in the same farm）. These data suggest that YNE could transmit from mallard duck to Yangjiang goose.
出处
《病毒学报》
CAS
CSCD
北大核心
2017年第6期892-897,共6页
Chinese Journal of Virology
基金
国家自然科学基金(项目号:31560707)
题目:新型麻鸭源禽痘病毒生物学特性和致病性研究
国家自然科学基金(项目号:31360601)
题目:羊痘病毒N1蛋白对宿主细胞凋亡
NF-κB和IRF3信号通路调控作用的研究
广西第18批十百千人才工程第二层次人选专项
题目:新型麻鸭源禽痘病毒生物学特性和致病性研究~~
关键词
禽痘病毒(APV)
水禽
鉴定
分型
Avipoxvirus（APV）
Waterfowl
Identication
Phylogenetic analyses
