贵州西南部艾纳香内生真菌多样性研究

Diversity of endophytic fungi from Blumea balsamifera in southwest Guizhou

本文以研究不同地点、时期和组织部位的艾纳香内生真菌类群多样性及分布为目标,从艾纳香Blumea balsamifera根、茎和叶中分离得到内生真菌152株,经形态分类学和分子生物学鉴定分别归属于11个属,其中Fusarium、Aspergillus、Trichoderma和Curvularia为艾纳香内生真菌的优势种群。结果表明,叶片内生真菌类群丰富度和类群多样性最高;罗甸、望谟、册亨和贞丰艾纳香内生真菌的分布比较集中,其中罗甸内生真菌类群多样性和类群丰富度最高;7月份在内生真菌类群多样性和类群丰富度上表现突出;而12月份分离获得的内生真菌菌株数量较多;不同地点、不同时期内生真菌分布相似程度较高,茎、根与叶的相似程度差别较大。

A total of 152 strains of endophytic fungi were isolated from roots, branches and leaves of Blumea balsamifera. Based on morphological characters and ITS rDNA sequence analysis, 11 genera were identified, of which Fusarium, Aspergillus, Trichoderma and Curvularia were dominant. The leaves possessed the highest diversity and population. Luodian, Wangmo, Ceheng and Zhenfeng prefectures showed relatively higher in species diversity and population, especially Luodian. The highest species richness occurred in July, while the highest isolation frequency in December. The distribution of endophytic fungi seems comparatively similar in different localities and growing periods, whereas distribution similarity in branches, roots and leaves is quite different.