野生型糙皮侧耳Pleurotus ostreatus JY2746对合成染料脱色性能的评价（英文）

Evaluation of the capacity to decolorize synthetic dyes by the wild strain JY2746 of Pleurotus ostreatus

随着我国印染工业的不断发展,人工合成染料给环境造成严重污染,现急需开发一种成本低廉、脱色效果显著的方法治理水污染问题。本研究发现糙皮侧耳的胞外粗酶液对5种合成染料均具有良好的脱色作用,其中对结晶紫、酸性品红和考马斯亮蓝G-250(浓度为40mg/L)的最高脱色率可分别达到100%、98.67%和92.5%。糙皮侧耳在液体发酵过程中主要产生3种酶类,即漆酶、锰过氧化物酶和木质素过氧化物酶,他们分别在第12天,第7天和第8天酶活达到最高,并且第12天产生的粗酶液对染料的脱色效果最好,根据酶活高峰形成时间与脱色率之间的关系,推测糙皮侧耳分泌的漆酶对染料起主要降解作用,而锰过氧化物酶和木质素过氧化物酶起辅助作用。本研究发现发酵粗酶液较纯化的酶类工艺简单,成本低廉,因此糙皮侧耳发酵液中提取的粗酶液在染料废水脱色处理方面具有潜在的应用价值。

With the development of printing and dyeing industry in China, dyes have become a problematic class of pollutants to the environment. Exploration of a method which is cheap and effective to control dye-polluted water resources is extremely urgent. In the present study, the extracellular crude enzyme from Pleurotus ostreatus showed excellent activities to remove five synthetic dyes from water. The decolorization rates for crystal violet, acid fuchsin and Coomassie brilliant blue G-250 solutions （40mg/L） were 100%, 98.67%, and 92.5%, respectively. Three kinds of enzyme, viz. laccase, manganese peroxidase and lignin peroxidase were generated from the fermentation of P. ostreatus. Their maximum enzyme activities were measured on 12th, 7th and 8th day. The decolorization rate was the highest when the crude enzyme extract of the 12th day fermentation was employed. According to the relationship between the date of maximum enzyme activity and decolorization rate, we infer that laccase plays a main role in the decolorization process, while manganese peroxidase and lignin peroxidase play supporting role. The current method indicated that employment of crude enzyme was a simpler and cheaper way than that of pure enzyme for decolorizing synthetic dyes. The crude enzyme from P. ostreatus was found to be a potentially applicable decolorizer for effective treatment of effluent.