蔗糖、柠檬酸、碳酸氢钠对3种口腔粘接剂粘接强度的影响

The influences of sucrose,citric acid and sodium bicarbonate on the adhesion of 3 kinds of adhesives

目的:研究3种粘接剂粘接强度受蔗糖、柠檬酸、碳酸氢钠的影响。方法:将60颗制备后的离体牙与60个氧化锆块(3 mm×3 mm×3 mm)随机分为3组:PULPDENT组、3M ESPE Relyx^(TM)Veneer组和Relyx^(TM)Luting组,每组再分为4小组(n=5):A组:人工唾液组;B组:10%蔗糖组;C组:0.2%柠檬酸组;D组:0.03%碳酸氢钠组。将试件用相应粘接剂粘接完成后,B、C、D组分别浸泡在蔗糖、柠檬酸和碳酸氢钠溶液中,2次/d,5 min/次,其余时间同A组一样浸泡在人工唾液中。3个月后,测定剪切粘接强度,20×显微镜观察断裂模式并在扫描电镜下观察断裂面。将数据用SPSS 17.0软件进行统计分析。结果:PULPDENT组、3M ESPE Relyx^(TM)Veneer组的粘接强度高于Relyx^(TM)Luting组(P<0.05);B组、C组、D组粘接强度低于A组(P<0.05);其余各组两两比较差异无统计学意义(P>0.05);3组粘接剂试件均显示为界面破坏;扫描电镜结果显示,Relyx^(TM)Luting组中,B组、C组、D组与A组相比龟裂纹深度、宽度及长度均增加;其余各组与对照组相比无显著区别。结论:相对于PULPDENT和3M ESPE Relyx^(TM)Veneer粘接剂,Relyx^(TM)Luting易受蔗糖、柠檬酸和碳酸氢钠的影响,不适用于氧化锆的粘接。

Objective： To study the influences of sucrose, citric acid and sodium bicarbonate on the adhesion of 3 kinds of adhesives. Methods： 60 extracted tooth and 60 zirconia blocks （3 mm × 3 mm×3 mm） were randomly divided into 3 groups（ n = 20）, namely PULPDENT group, 3M ESPE RelyxTM Veneer group and RelyxTM Luting group. Then, the samples of each group fell into 4 subgroups（ n =5）, namely subgroup A for artificial saliva, subgroup B for 10% sucrose, subgroup C for 0.2% citric acid, and subgroup D for 0.03 % sodium bicarbonate. After completing the adhesion of the specimens with corresponding adhesives, the specimens of subgroups A, B, C and D were submerged into artificial saliva（the control）, sucrose, citric acid and sodium bicarbonate solutions for 2 times/day and 5 min/time, respectively. For the rest of time, all the specimens were submerged in artificial saliva. 3 months later, shear bond strength of the specimens was tested, the fracture surface was observed under SEM, 20 × microscope, and the fracture model was observed by stereoscopic microscope. SPSS 17.0 software was adopted for statistical analysis of the data. Results： The bond strength of PULPDENT, 3M ESPE RelyxTM Veneer groups were higher than that of RelyxTM Luting group（ P 〈 0.05 ） ; the bond strength of subgroups B, C and D was lower than that of subgroup A（ P 〈 0.05 ） ; and the difference between the remaining groups was not statistically signifi- cant （P 〉 0.05 ）. SEM observation displayed that in group RelyxTM Luting, subgroups B, C and D showed increased crack depth, width and length when compared with subgroup A; there was no obvious difference between the remaining groups and the control group; in groups PULPENT,3M ESPE RelyxTM Veneer and RelyxTM Luting, all samples in their subgroups showed interface failure. Conclusion： Compared with PULPDENT and 3M ESPE RelyxTM Veneer adhesive, RelyxTM Luting is more susceptible to the influence of sucrose, citric acid and sodium bicarbonate, so it is not suitable for bonding zirconia blocks.