miR-186-5p对绵羊黑色素细胞迁移增殖的影响

The Influences of miR-186-5p on Migration and Proliferation of Sheep Melanocytes

旨在研究miR-186-5p对绵羊黑色素细胞迁移增殖的影响。本研究通过荧光定量PCR检测不同毛色绵羊皮肤中miR-186-5p的表达差异;运用细胞转染使miR-186-5p在绵羊黑色素细胞中过表达,然后通过荧光定量PCR检测miR-186-5p的表达,通过分光光度计测定转染细胞中黑色素含量,运用划痕试验判定黑色素细胞的增殖迁移能力。结果显示,miR-186-5p在绵羊不同毛色皮肤中均有表达,但白色皮肤表达高于黑色皮肤且差异显著(P<0.05);在过表达miR-186-5p的绵羊黑色素细胞中,miR-186-5p的表达显著升高(P<0.01);黑色素含量显著减少(P<0.05);黑色素细胞的增殖和迁移能力有所降低。综上表明,miR-186-5p与绵羊皮肤黑色素的生成有关,绵羊黑色素细胞中过表达miR-186-5p降低了黑色素的生成,同时也抑制了绵羊黑色素细胞的迁移和增殖。

The aim of this study was to explore influence of miR-186-5p on migration and proliferation of sheep melanocytes. The expression of miR-186-Sp in different color skin of sheep were determined by Quantitative real-time PCR; The over-expression vectors of miR-186-5p were transfected into sheep melanocytes, and then, the expression of miR-186-Sp was analyzed using Quantitative real-time PCR; The melanin content were detected by spectrophotometer; The capacity of migration and proliferation were analyzed by wound-healing assay. The results showed that the expression of miR-186-5p was significantly higher in white skin than that in black skin （P% 0.05）; In transfected melanocytes with over-expression of miR-186-Sp, the expression of miR- 186-5p was significantly increased （P^0.01）, the level of melanin content was significantly re- duced（P%0.05） ,the capacity of migration and proliferation of melanocytes also reduced. In summary, miR-186-5p was associated with the formation of melanin in sheep skin, in sheep melanocytes, overexpression of miR-186-5p would reduce the melanin production and suppress the migration and proliferation of melanocytes.