蒙药五根油剂对D-半乳糖致亚急性衰老模型小鼠的抗衰老作用研究

Study on Anti-aging Effect of Mongolian Medicine Wugen Youji(蒙药五根油剂) on Subacute Aging Model Mice Induced by D-galactose

目的:研究蒙药五根油剂的抗衰老作用。方法:将60只昆明小鼠(雌雄各半)随机分为5组,即正常组、模型组、蒙药五根油剂低剂量组和高剂量组、维生素E组,除正常对照组外,其余各组均采用颈背部皮下注射D-半乳糖,150 mg/(kg·d),建立亚急性衰老模型,同时分别灌胃给药蒙药五根油剂低剂量[0.8 g/(kg·d)]、高剂量[3.2 g/(kg·d)]和维生素E[0.86 mg/(kg·d)]。连续给药7周后处死动物,测定血清和脑组织中的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-px)及丙二醛(MDA)含量变化;检测脾脏和胸腺指数变化。结果:蒙药五根油剂各给药组可显著提高衰老小鼠血清及脑组织中的SOD、GSH-px含量,降低MDA含量;降低脾脏指数和胸腺指数,其中高剂量组的作用优于低剂量组。结论:蒙药五根油剂可明显提高衰老小鼠抗氧化能力,具有抗衰老作用。

Objective： To study the anti-aging effect of Mongolian medicine Wugen Youji.Methods： The 60 Kunming mouse（half male and female） were randomly divided into 5 groups： normal group,model group,Mongolian medicine Wugen Youji low dose group and high dose group,vitamin E group.Except the normal control group,the other groups were subcutaneously injected with D-galactose,150 mg/（kg·d）,to establish subacute aging model,and intragastric administration of Mongolian medicine Wugen Youji low dose group,high dose group and positive control group of vitamin E,dosage was 0.8 g/（kg·d）,3.2 g/（kg·d）,0.86 mg/（kg·d）.Sacrificed after administration of 7 consecutive weeks,detect content change of superoxide dismutaseb（SOD）,glutathione peroxidase（GSH-px） and malondialdehyde（MDA） in serum and brain tissues; changes of spleen and thymus index.Results： Mongolian medicine Wugen Youji each dose group can significantly improve the content of SOD and GSH-px,decreased MDA in serum and brain tissues; decreased the spleen index and thymus index,the effect of high dose group is better than the low dose group.Conclusion： Mongolian medicine Wugen Youji can significantly improve the antioxidant capacity of aging mouse,indicate that has anti-aging effect.