葡萄籽总酚的提取及其抗氧化活性评价

Total phenolic extract of grape seed and antioxidant activity evaluation

本文通过比较几种不同提取方法对葡萄籽总酚进行了提取,进一步对葡萄籽总酚的含量进行了测定,并通过DPPH自由基清除率的测定、FRAP总抗氧化能力评价了几种提取方法葡萄籽总酚的抗氧化能力。实验结果表明,超声波辅助提取法葡萄籽总酚提取物提取率最高,达到52.68%,总酚含量为57.40mg GAE·g^(-1)。抗氧化实验表明超声波辅助提取法葡萄籽总酚的DPPH清除率较高,IC_(50)值为0.13倍葡萄籽总酚提取液,FRAP总抗氧化能力在总酚原液条件下达到175.84μm FeSO_4。本研究为葡萄籽资源的开发和综合利用提供了基础。

In this paper, the total phenol extract of grape seed were extracted by different methods, and the total phenol content was determined. The antioxidant capacity of grape seed total phenol extract was evaluated by DPPH free radical scavenging and FRAP total antioxidant capacity tests. The results showed that ultrasonic assisted extraction method has the highest yield reached 52.68%, the total phenol content was 57.40 GAE mg·g^-1 Antioxidant tests showed that ultrasonic assisted extraction of grape seed total phenol I）PPH scavenging rate is highest, the IC50 value was 0.13 times concentration of grape seed total phenol extract. FRAP total antioxidant capacity value was 175.84μm FeSO4 .This study provides the basis on the development and comprehensive utilization of grape seed resources.