RBM8A在人结肠癌细胞株中的表达及其意义

RBM8A expression and its role in human colon carcinoma cell lines

目的检测RBM8A在不同结肠癌细胞株中的表达,为进一步体外实验筛选靶细胞。方法采用实时定量反转录聚合酶连锁反应(q RT-PCR)法以及蛋白免疫印迹(Western-blot)法检测RBM8A在结肠癌细胞株SW480、SW620、HT29、LOVO、COLO320DM、T84及人正常结肠上皮细胞株NCM460中的表达。结果 RBM8A在7株细胞株中均有表达,其中在结肠癌细胞株SW620和COLO320DM中的表达高于正常细胞株,差异有统计学意义(P<0.05),而其余细胞株中RBM8A的表达与正常细胞株差异无统计学意义(P>0.05)。结论 RBM8A在结肠癌细胞株SW620和COLO320DM中高表达,SW620和COLO320DM细胞株或可作为靶细胞。

Objective To detect RBM8A expression in human colon carcinoma cell lines in order to select the most appropriate cell line for further study. Methods RBM8A expression was examined in the following cell lines using quantitative RT-PCR and Westernblotting： SW480, SW620, HT29, LOVO, COLO320DM,T84,and NCM460 enterocytes. Results RBM8A mRNA and protein were detected in all seven cell lines. RBM8A expression was higher in SW620 and COLO320DM cell lines than in normal enterocytes （P〈0.05）. Expression was similar between the normal enterocyte cell line and other cell lines （P〉0.05）. Conclusion RBM8A expression is higher in SW620 and COLO320DM eell lines, so they may be more suitable for more extensive laboratory studies.