CRISPR/Cas9基因编辑技术对抗病毒潜伏感染的应用进展

Recent progress in CRISPR/Cas9-mediated genome editing of viruses against latent infection

目前抗病毒治疗主要靶向产毒阶段的病毒蛋白酶系统,而对于具有潜伏感染特征的病毒无效,因此能够靶向病毒基因组的抗病毒药物将是未来对抗病毒潜伏感染的研究策略.CRISPR/Cas9作为一种近年快速发展的基因编辑技术,在对抗病毒潜伏感染方面取得一定的成果,如HIV、人致病疱疹病毒、HBV等.此文对其应用进展进行综述,并总结了逃逸突变体的产生、脱靶效应、载体安全性等问题,以期为研制有效的抗潜伏感染病毒的治疗方法提供参考依据.

Current antiviral therapy mainly targets the viral proteins and proteases system in high-level viral replication stage,but is ineffective in latent infected viruses.Therefore,antiviral medicine targeted viral genome will be the future research strategies against latent infections.CRISPR/Cas9,as a rapidly developed genome editing technology,has been made some achievements against virus latent infections,such as HIV,human herpes,HBV,etc.This review introduces the latest research results of CRISPR/Cas9 technology against virus latent infections,and summarizes issues such as immune escape mutants,off-target effect and carrier safety,so as to provide references for researching effective treatment.