乌司他丁对重症哮喘患者血浆中CRP、IL-8、IL-10、TNF-α水平的影响

Effect of Ulinastatin in the level of plasma inflammatory cytokines, CRP, IL-8, IL-10 and TNF-α, of patientswith severe asthma

目的探讨乌司他丁对重症哮喘患者血浆中C反应蛋白（CRP）、IL-8、IL-10、肿瘤坏死因子α（TNF—α）水平的影响。方法纳入我院2016年3月至2017年3月就诊住院的90例重症哮喘患者，随机分成实验组和对照组，各45例。两组患者均同时给予常规治疗，如吸氧、抗炎、雾化吸入、激素治疗等，实验组在上述常规治疗上加用乌司他丁治疗10d。收集外周血采用酶联免疫吸附法（ELISA）和散射比浊法检测血浆中CRP、IL-8、IL-10、TNF-α炎性细胞因子水平。分析重症哮喘患者常规治疗中加用乌司他丁治疗后血浆中炎性细胞因子水平的变化。结果2组患者治疗后血浆中CRP、IL-8、TNF-α炎性细胞因子水平与治疗前相比均明显降低（P值均〈0．05），而IL-10炎性细胞因子水平与治疗前相比显著升高（P〈0．05）。治疗后实验组CRP、IL-8、TNF-α水平低于对照组（P值均〈0．05），而IL-10炎性细胞因子水平显著高于对照组（P〈0．05）。结论乌司他丁对重症哮喘患者治疗有效，可以显著降低CRP、IL8、TNF-α血浆炎性细胞因子水平，并升高IL-10炎性细胞因子水平，可用做重症哮喘患者治疗中的一种辅助性治疗药物。

Objective To discuss the effect of ulinastatin in the level of plasma inflammatory cytokines,C reactive protein （CRP）, IL-8, IL-10 and tumor necrosis factor （TNF-α）, of patients with severe asthma. Methods The paper included 90 patients with severe asthma were randomly divided into the experimental group and the control group, who visited hospital to seek medical treatment from March 2016 to March 2017. Two groups of patients were given routine treatment, such as oxygen, anti- inflammatory, inhalation, hormone therapy, experimental group treated with 10 days. of ulinastatin in the treatment, collected peripheral blood samples, detected the level of CRP, IL-10, TNF-α and other inflammatory cytokines in plasma by adopting enzyme linked immunosorbent assay （ELISA） and scatter turbidimetry. Analysis of changes of inflammatory cytokines in plasma after treatment with ulinastatin in patients with severe asthma treatment. Results Two groups of patients after treatment, the level of CRP, IL 8, TNF-α were decreased significantly compare with before treatment （ P 〈0.05）, and the level of IL- 10 increased significantly compared with those before treatment （ P 〈0.05）. After treatment, the level of CRP, IL-8, TNF-α with experimental group were lower than the control group （ P 〈0.05）, and the level of IL-10 were higher than the control group （ P〈 0.05）. Conclusions Effect of ulinastatin on the treatment of patients with severe asthma effectively, can significantly reduce the inflammatory cytokines level of CRP, IL-8, TNF-α, and elevated the inflammatory cytokines level of IL-10, can be used as an adjunctive therapy in drug treatment in patients with severe asthma.