非小细胞肺癌患者外周血Th1、Th2、Th17淋巴细胞及其相关细胞因子表达及临床意义

Clinical Signification and expression of Th1、Th2、Th17 lymphocyte and related cytokine in peripheral blood of patients with non-small cell lung cancer

目的:观察非小细胞肺癌(NSCLC)患者外周血中Th1、Th2、Th17淋巴细胞及其细胞因子IFN-γ、IL-4、IL-17的表达及临床意义。方法:收集山西省肿瘤医院胸外科,原发未治疗的NSCLC60例患者作为实验组和60例健康志愿者作为对照组,以IFN-γ、IL-4、IL-17为代表分别测定Th1、Th2、Th17淋巴细胞因子。首先采用流式细胞技术(FCM)分别检测两组外周血Th1、Th2、Th17淋巴细胞含量,采用酶联免疫吸附法(ELISA)分别检测两组血清中细胞因子IFN-γ、IL-4、IL-17水平含量。同时分析比较两组外周血Th1、Th2、Th17淋巴细胞及细胞因子IFN-γ、IL-4、IL-17水平含量与临床病理特征关系。结果:实验组外周血Th1、Th2、Th17淋巴细胞的水平分别为(44.57±20.32)%、(3.08±1.28)%和(1.74±1.18)%;对照组外周血中Th1、Th2、Th17淋巴细胞的水平分别为(49.04±12.30)%、(2.67±1.36)%和(0.89±0.56)%,两组比较差异有统计学意义(P<0.05)。Ⅲ、Ⅳ期肺癌患者Th1淋巴细胞的水平(41.1±23.6)%、(44.6±20.3)%低于Ⅰ—Ⅱ期肺癌患者(46.2±19.1)%(P<0.05),Ⅲ、Ⅳ期肺癌患者的Th2、Th17淋巴细胞水平高于Ⅰ—Ⅱ期肺癌患者(P<0.05)。鳞癌患者外周血Th2、Th17淋巴细胞水平高于腺癌患者。结论:检测NSCLC患者外周血Th1、Th2、Th17及其细胞因子IFN-γ、IL-4、IL-17水平含量对判断病情、了解机体的免疫功能具有重要的临床意义。

Objective： To study the expression and its clinical significance of Th1 ,Th2 ,Th17 lympho- cyte and IFN- γ,IL-4,IL-17 related eytokine in peripheral blood of patients with non-small cell lung cancer （NSCLC）. Methods： 60 patients with primary and untreated NSCLC were enrolled and designed as experimental group,at the same time, 60 healthy volunteers as control group. IFN-γ、IL-4,IL-17 were used as markers for the differentiation of Th1 ,Th2 、Th17 lymphocyte subsets, respectively. Flow eytometry （ FCM ） was used for detecting the level of Th1 ,Th2,Th17 lymphocyte of 2 groups. Enzyme linked immunosorbent assay （ELISA） was used for detecting the level of IFN- γ,IL-4 ,IL-17 cytokine of 2 groups. Compared the levels of Thl ,Th2, Thl7 lymphocyte and IFN- γ ,IL-4 ,IL-17 cytokinc in peripheral blood of patients and analyzed two grounp relationship with clinieopathologieal characteristics. Results： The levels of Th1 ,Th2 and Th17 lymphocytes in the peripheral blood of the experimental group were （ 44. 57 ± 20. 32 ） %, （ 3.08 ± 1.28 ） % and （ 1.74 ± 1.18 ） %,respectively; while those from the control group were （ 49.0 ±12. 3 ） %, （ 2. 7 ±1.4 ） % and （ 0. 9 ±0.6 ） %. There were significant difference in Th1 ,Th2,Th17 lymphocyte and IFN- γ,IL-α,IL-17 cytokine between patients with stage Ⅲ,Ⅳ and those stage Ⅰ-Ⅱ （P 〈 0. 05）. Conclusion： The detection of Th1 ,Th2,Th17 lymphocyte and the levels of cytokines IFN- γ,IL-4,IL-17 in peripheral blood of patients with NSCLC have important clinical significance in judging the disease condition and understanding the immune function of the body.