EB病毒microRNA Bart6-5p表达对鼻咽癌上皮间质转化的影响

Role of EBV microRNA Bart6-5p in the epithelial-mesenchymal transition in nasopharyngeal carcinoma

目的探讨EB病毒micro RNA Bart6-5p表达对鼻咽癌细胞上皮间质转化(epithelial mesenchymal transition,EMT)的影响。方法采用实时荧光定量PCR检测EB病毒阳性的鼻咽癌细胞株C666-1和淋巴瘤细胞株Akata、Daudi、Raji及Namalwa中micro RNA Bart6-5p和Dicer m RNA的表达,在恒定携带EB病毒且micro RNA Bart6-5p表达最高的鼻咽癌细胞株C666-1中抑制micro RNA Bart6-5p表达后检测Dicer和与鼻咽癌EMT相关分子标志物E-cadherin、Vimentin、ZEB1和ZEB2 m RNA的表达。结果 EB病毒阳性细胞株中micro RNA Bart6-5p m RNA表达水平按C666-1、Akata、Daudi、Raji及Namalwa顺序依次递减(P<0.05),而Dicer m RNA表达水平则依次递增(P<0.05)。在恒定携带EB病毒且micro RNA Bart6-5p表达最高鼻咽癌细胞株C666-1中抑制micro RNA Bart6-5p表达后,Dicer m RNA表达呈升高趋势(P<0.05),与鼻咽癌EMT相关分子标志物E-cadherin m RNA表达呈升高趋势(P<0.05),Vimentin、ZEB1及ZEB2m RNA表达则呈下降趋势(P<0.05)。结论 EB病毒micro RNA Bart6-5p可能通过调控Dicer基因表达而进一步影响鼻咽癌的上皮间质转化过程。

Objective To investigate the ability of Epstein-Barr virus （EBV） microRNA Bart 6-5p to regulate Dicer expression and thereby ilffluence the epithelial-mesenchymal transition（EMT） in nasopharyngeal carcinoma cells. Methods Expression of microRNA Bart6-5p and Dicer were quantitated using real-time quantitative PCR in the EBV-positive nasopharyngeal carcinoma cell line C666-1 and lymphoma cell lines Akata, Daudi, Raji and Namalwa. In C666-1 cells with the highest EBV load, microRNA Bart6-5p expression was inhibited and subsequent changes in levels of EMT-related molecular markers were examined. Results Bart6-5p expression followed the trend C666-1 〉Akata〉Daudi〉Raji 〉Namalwa, while Dicer followed the inverse trend. Inhibiting Bart6-5p expression in C666-1 increased expression of Dicer and the positive EMT marker E-cadherin,while it decreased expression of the negative EMT markers vimentin,ZEB1 and ZEB2. Conclusion EBV mieroRNA Bart6-5p can influence the EMT in nasopharyngeal carcinoma by regulating Dicer expression.