正常菌与可吸入矿物细颗粒作用致突变性研究

A study of the mutagenicity of normal bacteria interacted with inhalable mineral granules

采用Ames试验分别对蒙脱石、石英、方解石、钠长石4种可吸入矿物细颗粒及其与大肠杆菌、表皮葡萄球菌相互作用48 h后的混悬液作为受试物进行检测,分析IMG是否具有致突变性,及其与人体正常细菌群作用后,IMG/正常菌复合体是否有致突变性毒力改变。结果显示石英以及石英/大肠杆菌复合体对TA98、TA100菌株Ames试验呈阳性反应;石英/表皮葡萄球菌复合体对TA98菌株Ames试验呈阳性反应;而蒙脱石、方解石、钠长石纯矿及其与正常菌作用后的复合体Ames试验结果均为阴性。结果表明石英具有直接致突变作用;石英与正常菌菌作用后复合体依然具有致突变性,但石英表现出的碱基置换突变作用在与表皮葡萄球菌作用后消失,其机制有待进一步研究。

The mutagenicity of inhalable mineral granules （IMG） and interaction with Epidermis staphylococ- cus, E. coli were studied by Ames test to analyze whether IMG and IMG/body normal flora complex has muta- genicity. The results show that Ames test of the quartz and quartz/E, coli complex on TA98 and TA100 strains assumes positive reaction, Ames test of quartz/epidermis staphylococcus complex on TA98 strains assumes posi- tion reaction, whereas Ames test of montmorillonite, calcite, albite and complex from interaction with body nor- mal flora exhibits negative reaction, which suggests that the quartz has direct mutagenic effect, quartz/body normal flora complex still has mutagenicity, but the mechanism of the phenomenon that base replacement muta- tion effects of quartz disappeared after interaction with Epidermis staphylococcus needs further research.