人体几种典型病理性钙化物中碳羟磷灰石的红外光谱研究

An infrared spectrum analysis of several kinds of typical pathologic calcification product in human body

选取人体心脏瓣膜、动脉、甲状腺、卵巢和乳腺5大类典型病理性钙化共38例样品开展了系统的红外光谱表征,重点探讨了样品内碳羟磷灰石(CHA)中CO2-3含量及CO2-3的取代类型特征。研究发现,这5类典型病理性钙化产物均以碳羟磷灰石为主要物相,相同部位钙化物中的CO2-3含量基本保持一致,但不同部位钙化物中的CO2-3平均含量差别较明显,甲状腺、乳腺中CO2-3平均含量最低,为6.2%,相比CO2-3平均含量最高的心脏瓣膜,相差3.3%。5类样品含有的碳羟磷灰石均以B型取代(CO2-3取代PO3-4)为主,伴随有少量A型取代(CO2-3取代OH-),钙化物中的CO2-3含量与B/A取代比值的正向联系不明显。

In this study, the human pathologic calcification samples were selected from human heart valve, artery, thyroid, ovarian and breast. Focusing on 38 cases of five different types, the authors carried out a systematic infrared spectrum characterization and, with the help of infrared spectrum method, inferred the presence of the carbonate group and evaluated the carbonate substitution in bioapatite structure. It was found that the basic composition of the five kinds of typical pathologic calcification product was carbonate-hydroxylapatite （CHA）. The carbonate content of the samples in the same part remained the same,but the differences of the average content of the carbonate in different parts were obvious. The average content of CO32- in the thyroid as well as the breast was 6.2, 3.3% lower than that in the heart valve. The average content of CO32- in the heart valves was the highest. The infrared spectra showed that the pathological bioapatite mainly was the B-type carbonate substitution （CO32- for PO43-）, accompanied by a small amount of A-type carbonate substitution （CO32- for OH-）. The forward contact of the carbonate content inferred by the spectroscopic measurements and the ratio of B/A substitution was unconspicuous.