针刺结合雷火灸对膝骨关节炎大鼠MMP-3、TIMP-1及TGF-β1的影响（英文）

Effect of acupuncture plus thunder-fire moxibustion on MMP-3, TIMP-1 and TGF-β1 in rats with knee osteoarthritis

目的:观察针刺结合雷火灸对膝骨关节炎(KOA)大鼠软骨组织中基质金属蛋白酶-3(MMP-3)、基质金属蛋白酶抑制剂-1(TIMP-1)和转化生长因子-β1(TGF-β1)表达的影响,探讨针刺结合雷火灸治疗KOA的作用机制。方法:Sprague-Dawley(SD)大鼠30只,根据随机数字表法随机分成空白组、模型组和针灸组,每组10只大鼠。模型组和针灸组予木瓜蛋白酶右后膝关节注射造模。治疗2星期后,采用酶联免疫吸附法测定各组大鼠滑膜MMP-3、TIMP-1的含量,采用Motic B5显微摄像系统测定TGF-β1的含量。结果:模型组软骨组织中MMP-3和TIMP-1的含量较空白组显著增加(均P<0.01);针灸组MMP-3和TIMP-1含量低于模型组,组间差异有统计学意义(均P<0.05);针灸组MMP-3和TIMP-1含量高于空白组,组间差异有统计学意义(均P<0.05)。模型组软骨组织中TGF-β1的含量较空白组显著降低(P<0.01),针灸组TGF-β1含量较模型组升高(P<0.05),但低于空白组,组间差异有统计学意义(P<0.05)。结论:针刺结合雷火灸能有效平衡KOA模型大鼠中MMP-3和TIMP-1的异常表达,并且对TGF-β1具有一定的上调功能,这可能是针刺结合雷火灸治疗KOA的作用机制之一。

Objective： To observe the effect of acupuncture plus thunder-fire moxibustion on the expressions of matrix metalloproteinase-3 （MMP-3）, tissue inhibitor of metalloproteinase-1 （TIMP-1） and transforming growth factor-β1 （TGF-β1） in cartilage of knee osteoarthritis （KOA） rats, and to explore the mechanism of acupuncture plus thunder-fire moxibustion in the treatment of KOA. Methods： Thirty Sprague-Dawley （SD） rats were randomly divided into a blank control group, a model group and an acupuncture-moxibustion group by random digits table, 10 rats in each group. Rats in the model group and the acupuncture-moxibustion group were injected with papain in the right posterior knee joint to prepare the models. The levels of MMP-3 and TIMP-1 in rat synovium of each group were measured by enzyme-linked immunosorbent assay （ELBA） after 2 weeks of treatment. The level of TGF-β1 was determined by Motic B5 Micro-camera system. Results： The levels of MMP-3 and TIMP-1 in the cartilage of the model group were significantly higher than those in the blank control group （all P〈0.01）; the levels of MMP-3 and TIMP-1 in the acupuncture-moxibustion group were lower than those in the model group, and the between-group differences were statistically significant （all P〈0.05）. The levels of MMP-3 and TIMP-1 in the acupuncture-moxibustion group were higher than those in the blank control group, and the differences were statistically significant （all P〈0.05）. The level of TGF-β1 in cartilage tissues of the model group was significantly lower than that in the blank control group （P〈0.01）; the level of TGF-β1 in the acupuncture-moxibustion group was higher than that in the model group （P〈0.05）, but it was lower than that in the blank control group, and the between-group difference was statistically significant （P〈0.05）. Conclusion： Acupuncture plus thunder-fire moxibustion can effectively recover the abnormal expressions of MMP-3 and TIMP-1 in KOA model rats and somewhat up-regulate TGF-β1, which may be one of its mechanisms of acupuncture plus thunder-fire for KOA.