磷酸化蛋白激酶和人类端粒反转录酶在上皮性卵巢癌中的表达及临床意义

Expressions and clinical significance of pAktl and hTERT in epithelial ovarian carcinoma

目的探讨磷酸化蛋白激酶B1（pAkt1）和人类端粒反转录酶（hTERT）在上皮性卵巢癌中的表达及其对预后的影响。方法采用免疫组织化学SP法检测72例上皮性卵巢癌组织和10例正常卵巢组织中hTERT和pAkt1蛋白的表达，分析pAkt1、hTERT蛋白表达的相关性及其与临床病理特征和临床预后的关系。 结果上皮性卵巢癌组织pAkt1阳性表达率为73.6%，hTERT阳性表达率为56.9%，与正常卵巢组织相比差异均有统计学意义（χ2=20.814,P〈0.001; χ2=11.389,P〈0.001）。pAkt1、hTERT蛋白在上皮性卵巢癌中表达无关（r=0.075,P=0.532）；pAkt1、hTERT蛋白表达与患者肿瘤病理分化程度相关（χ2=6.475,P=0.011;χ2=1.370，P=0.001）；pAkt1和hTERT蛋白同时阳性表达的患者无瘤生存期明显短于非共同表述患者（8.585个月∶11.227个月，χ2=4.361，P=0.037），但总生存期差异无统计学意义（11.107个月∶11.695个月, χ2=0.394,P=0.530）。结论pAkt1和hTERT蛋白在上皮性卵巢癌中高表达，pAkt1和hTERT蛋白同时高表达提示上皮性卵巢癌患者预后较差。

ObjectiveTo evaluate the expressions and prognostic significance of phosphorylation of protein kinase B1 （pAkt1） and human telomere reverse transcriptase （hTERT） in epithelial ovarian carcinoma. MethodsThe expressions of pAkt1 and hTERT were examined by immunohistochemical SP method in 72 cases of epithelial ovarian carcinoma and 10 normal endometrial tissues to analyze the correlation between pAkt1 and hTERT and their relationships with clinicpathological features and prognosis. ResultsThe positive expression rate of pAkt1 in epithelial ovarian carcinoma was 73.6%, and the positive expression rate of hTERT was 56.9%, compared with normal ovarian tissues, with statistical significance （χ2=20.814, P〈0.001; χ2=11.389, P〈0.001）.The expression of pAkt1 was not associated with hTERT expression （r=0.075, P=0.532） in epithelial ovarian carcinoma. The expressions of pAkt1 and hTERT in epithelial ovarian carcinoma were correlated with tumor differentiation status （χ2=6.475, P=0.011; χ2=1.370, P=0.001）. The diseasefree survival of patients with pAkt1 and hTERT positive coexpression was significantly shorter than others （8.585 months vs. 11.227 months, χ2=4.361, P=0.037）, but there was no significant difference in overall survival （11.107 months∶11.695 months, χ2=0.394, P=0.530）. ConclusionpAkt1 and hTERT are highly expressed in epithelial ovarian carcinoma, and high coexpression of pAkt1 and hTERT indicates poor prognosis in epithelial ovarian carcinoma.